The Good
Very sensitive detection of cell death.
The Bad
Quite a long protocol, some buffers need to be made just before use.
The Bottom Line
Overall the protocol is pretty straight forward but you need to read which buffers need to be made in advance and which ones on the spot, just before the next step is performed. Ensure you keep the fluorescent buffers covered in foil (and the ones being made at the beginning on ice). The manufacturer suggests quite a small volume of reagents for each coverslip, but surprisingly the volume is sufficient to perform the incubations, and its possible to see the light at the end of the TUNEL.