Clean Separation of Tumor Cells

December 14, 2018

BlueskyReddit
Johns Hopkins School of Medicine
Oncology
Lab Technician

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Company:

GE Healthcare Life Sciences

Product Name:

Percoll density gradient media

Catalog Number:

17089101

Our lab uses Percoll for occasions when we want a better separation between lymphocytes and monocytes. In these cases, we use Percoll over Ficoll. This requires multiple layers of density to achieve our layer of cells.

Experimental Design and Results Summary

Application

Cell isolation

Starting Material

Murine T cells

Protocol Overview

Since we want a clean lymphocyte population after harvesting tumors from mice, we layer it by 40% and 80% density gradient. We underlay the 80% on the bottom and spin in 3000 rpm for 45 minutes without brakes.

Tips

Make sure the Percoll is at room temperature, otherwise the separation will be extremely unclean.

Results Summary

We always rely on this method to get a clean yield of lymphocytes from tumors.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Clean and excellent yield

The Bad

Percoll must be at room temperature after storing at 4°C.

The Bottom Line

This is extremely useful for isolating lymphocytes. A must-buy for laboratories looking for ways to get a clean yield after harvesting tumors from mice.

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