Thermo Fisher Scientific
NanoDrop 2000
ND-2000
Info: Browse Small Volume Spectrometers View Product
Among many alternatives available, the NanoDrop 2000 is an all-purpose workhorse in the lab. It is a fantastic device to measure DNA and RNA concentrations in microvolumes (1 µl) if you are preparing samples that are not of sub-nanogram concentrations (< 10 ng/uL, do you need it in reality?). Measurements are easy, quick and of excellent quality. We all know that fluorometers (such as Qubit from Thermo or Quantus from Promega) offer superior concentration measurements for samples with < 10 ng/uL. However, these devices require buying kits and some preparation of the sample before reading. With the NanoDrop 2000, I pipette 1 uL of the sample and “voilà” - the results are on the screen. No further costs. Moreover, it allows connection to a PC for saving the data in many electronic formats.
Acid nucleic quantification
After blanking the machine, dispense 1.0 μl of the sample directly on top of the Nanodrop sensor. Lower the arm and make the reading
Use a kimwipe to clean the sensor and its metal cap to reduce contamination between samples.
Software integration
N/A
The quality of the measurements and the ease/speed of use.
Not accurate for samples concentrated with less than 5 ng/uL
Fantastic device!