Time Course Effect Of Chemoattractant On Acetylation Of PPARgamma

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Company:

Sigma Aldrich

Product Name:

Anti-acetylated lysine antibody

Catalog Number:

SAB5200090

One of the important protein modification in cell biology and proteomic studies is acetylation. It is one of the co-translational and post translational modifications of protein. Here we checked the acetylation of PPAR gamma by chemoattractant.

Experimental Design and Results Summary

Applications

Immunoprecipitation

Sample

Human lung cells

Primary Incubation

2 microgram overnight

Blocking Agent

5% milk with 1x TBST

Secondary Incubation

1:1000 dilution in 5% milk prepared in 1xTBST

Tertiary Incubation

1:4000 dilution in 5% milk prepared in 1xTBST

Detection

GAR

Results Summary

There is increased acetylation in a time dependent manner.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

This antibody is very good for IP experiment

The Bad

None

The Bottom Line

This antibody is very good for IP. Highly recommended.

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