Countess II FL Can Count, Can You?

Purdue University
CPB
Graduate Student

Overall

Performance

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Invitrogen

Product Name:

Countess II FL

Catalog Number:

AMQAX1000

We were considering an automated cell counting method for use with murine primary cells from spleen, lymph node, and digested intestinal tissues. We might occasionally use with transfected cells or cell cultures, but the bulk of our research is primary cells. Given the large volume of cell counts we perform, we didn't want an instrument that required purchasing consumables routinely, and the Countess II is able to use a glass hemocytometer or disposables.

Experimental Design and Results Summary

Tips

Coverslips scratch easily and scratches can interfere with cell counts.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Reproducible (sample-dependent), no consumable usage.

The Bad

Not good for some primary cells, scratches in coverglass interfere with counts, doesn't save that much time for cell counting.

The Bottom Line

We're not likely to purchase this after the demo. Overall, the time saved from automating was much less than we were led to believe (we've learned to be fast cell counters), and the benefits (mainly reproducibility) aren't worth the cost of purchasing and implementing this device.

Join the discussion