STEMCELL Technologies
Mouse Naive CD4+ T Cell Isolation Kit
19765A
I use this kit to isolate naive, murine CD4+ T cells from murine spleens for down-stream antigen-dependent, in vitro assays.
Cell Isolation
Murine Spleen
Single cell suspension at 10e8/mL is labelled through the manufacturer's protocol. Naive CD4+ T Cells are untouched as this is a negative selection.
Double incubation times listed.
This kit is very effective at purifying untouched, naive CD4+ T cells for down stream applications.
N/A
Ease of use, affordable compared to FACS
Takes longer than listed for efficient isolation.
Great kit if you are needing naive T cells that haven't been labelled for isolation.