A Good Flow Cytometer, But...

University of Kentucky
Toxicology
Postdoctoral Fellow

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Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

LSR-II

Catalog Number:

Special order BD LSR II

BD's LSR-II analyzer has the capacity to support up to 7 lasers and has 56 positional choices for selection of the detectors; however, the model I am reviewing has 3 lasers and 9 detectors. It is now ~ 8 years old, and it shows! One laser is nearly dead, and the machine needs regular maintenance.Yet, it is a reliable instrument most of the times and provides digital output (FCS 3.0 and 3.1 files). The software (Diva 6.0) is OK, but not the easiest to use. I think BD can learn a thing or two from FlowJo.

Experimental Design and Results Summary

Tips

You need to understand the basics of flow cytometry, otherwise you are wasting your time.

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Summary

The Good

Digital

The Bad

Moody

The Bottom Line

Newer, more modern machines are now available. You may be better off looking at those.

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