BD Biosciences
LSR-II
Special order BD LSR II
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BD's LSR-II analyzer has the capacity to support up to 7 lasers and has 56 positional choices for selection of the detectors; however, the model I am reviewing has 3 lasers and 9 detectors. It is now ~ 8 years old, and it shows! One laser is nearly dead, and the machine needs regular maintenance.Yet, it is a reliable instrument most of the times and provides digital output (FCS 3.0 and 3.1 files). The software (Diva 6.0) is OK, but not the easiest to use. I think BD can learn a thing or two from FlowJo.
Flow Cytometry
None
The machine is quite robust, even when the sample is poor. Priming and cleaning with 10% bleach usually unblocks the flow.
You need to understand the basics of flow cytometry, otherwise you are wasting your time.
Results are very much dependent on your assay. As I always say: "garbage in, garbage out". Make sure you prepare the samples properly including single-color compensation controls. Always talk to someone who has experience with the machine and the technique before you even run your assay!
Robust, durable, but needs maintenance
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Digital
Moody
Newer, more modern machines are now available. You may be better off looking at those.
Product Name: BD LSRSupplier Page