145-0010
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Our lab uses insect cell cultures to produce recombinant mammalian proteins. For each protein that is produced using this method, considerable attention must be paid to the density of the cell cultures at various stages in the protocol. A failure to accurately document the density of cells in a culture will lead to a reduction in the efficiency of protein production or worse, complete cell death. When generating proteins using this method we often have 5-6 individual cell cultures incubating at a time. As a result, manual counting of cells in each culture can be needlessly time consuming. We bought the TC-10 to cut down the time spent counting cells and to standardize our protocols such that different users were able to count cells without user bias.
When insect cells are clumped together, the instrument determines them to be just one cell. As a result, it is worth viewing the cells on the screen to make sure that they are not clumped and therefore skewing the count. Additionally, if there is a sudden and unexpected drop in the viability of the cells, it is worth repeating the count. Occasionally, the focus function of the machine is slightly off, leading the instrument to interpret out-of-focus cells as being dead cells.
Product Name: TC-10 Automated Cell CounterSupplier Page