HPLC UV-Vis Detectors

HPLC UV detectors are used with high performance liquid chromatography to detect and identify analytes in the sample. A UV visible HPLC detector uses light to analyze samples. By measuring the sample's absorption of light at different wavelengths, the analyte can be identified. HPLC UV detectors can be used by any lab using HPLC, including genomic, biology, and biochemistry laboratories, to analyze nucleic acids, proteins, and to do toxic and therapeutic drug testing. Two types of HPLC UV detectors are single and variable wavelength detectors. Single wavelength detectors measure the samples absorption of a single wavelength, while variable wavelength detectors measure absorption of multiple wavelengths and are therefore more sensitive. When deciding on a HPLC UV detector, consider whether a single wavelength will be sufficient or if a variable wavelength detector is needed. The wavelength range that can be used and the sensitivity of the detector should also be factored into the decision.