An HPLC detector is a crucial component of the high-performance liquid chromatography system, as it is responsible for identifying the analytes traveling through the column.
There are different types of detectors a researcher could use, which vary depending on the chemical and physical properties of the eluting samples.
This catalog lists a variety of HPLC detectors from different manufacturers.
Types of HPLC Detectors
- Diode Array Detector - These are most commonly used to record the ultraviolet and visible (UV-vis) absorption spectra. An advantage of the diode array detector is the ability to select the best wavelength for analysis.
- Conductivity Detector - These detectors measure the ability to pass an electric current by recording electronic resistance. This can be used to determine the concentration of ionic compounds.
- Evaporative Light Scattering Detector - ELSDs provide comprehensive information about analytes, including measurement of particle size below a nanometer, molecular weight, and zeta potential.
- Refractive Index Detector - HPLC RI detectors are often used when analyzing substances with limited or no UV absorption, which can include alcohols, sugars, fatty acids, polymers, and carbohydrates.
- UV-Vis Detector - These detectors measure the absorption of light at different wavelengths. Single wavelength detectors measure absorption of a single wavelength, while variable wavelength detectors can measure multiple wavelengths, improving sensitivity.
- Fluorescence Detector - These detectors introduce excitation light at a particular wavelength and measure the intensity of the emitted light at another wavelength. They can be used to measure concentrations of analytes such as pharmaceuticals and other fluorescent compounds.
Recommended Reading:
Analysis supports every stage of research and development, and for biomedicine that means high-performance liquid chromatography (HPLC). Although the process of selecting HPLC systems and detectors is unique for every circumstance and every life science laboratory, enough commonality exists to justify this guide.
Time-saving tools also help ensure reliable results
read more
Following the moving molecular target
read more
I routinely use CRISPR/Cas9-based methodology to generate gene knockouts in cell lines. For this purpose, I use a ribonucleoprotein (RNP) strategy with SpCas9 purified in-house. Previously, I purified His-tagged SpCas9; this time, I wanted to test a ...
read more
The Superdex 200 Increase is a column made from agarose-dextran that enables size-dependent separation of proteins. It effectively separates proteins based on their size and offers excellent resolution specifically in the 100-300 kDa range. This ...
read more