Fig 1: (a) All PDGFRβ substitutions (p.Asn666Lys, p.Asn666Ser, p.Asn666His, and p.Asn666Tyr) increase basal phosphorylation at 37°C. (b) Exposure to 32°C for 6 h further increases phosphorylation in the p.Asn666Tyr, p.Asn666Lys, and p.Asn666His substitutions. Total phosphorylated PDGFRβ levels were measured by ELISA. Nontransduced immortalized fibroblasts were referred to as “nt,” while fibroblasts transduced with PDGFRB wild-type vector were denoted as “WT PDGFRB.” (a) One-way ANOVA with Dunnett's test compared wild-type PDGFRB and variants at 37°C (⁣∗∗∗∗p < 0.0001). (b) Cells were incubated at 32°C or 37°C, and two-way ANOVA with Šídák's test compared phosphorylation between temperatures for each variant (⁣∗p < 0.05;∗∗∗p < 0.001;∗∗∗∗p < 0.0001).