Human IL-2 Quantikine ELISA Kit D2050 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemHuman IL-2 Quantikine ELISA Kit
CompanyR&D Systems, a Bio-Techne Brand
Price $680.00Supplier Page View Company Product Page
Catalog NumberD2050
Quantity1 Kit (also 1 Pack (50 Plates), 1 Pack (6 Plates))
ApplicationsELISA
Detection Range31.20 - 2000 pg/mL
ReactivityHuman
Sample TypeCell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Citrate Plasma (100 uL)
Sensitivity7 pg/mL
Detection TargetIL-2
StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date
NCBI Full Gene Nameinterleukin 2
NCBI Gene AliasesIL-2, TCGF, lymphokine

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Endoglin-Directed CAR T Cells Comprehensively Target Tumors in Advanced SarcomasCancer Immunology ResearchOctober 1, 2025Harrison R. Berger, Malina Maharana, Jeneffer Mirabal, Lyazat Kurenbekova, Alberto Delaidelli, Atreyi Dasgupta, Ahmed Z. Gad, Mohamed F. Sheha, Sybrina S. Kerr, Ada I. Ozcan, et al.used as controls. The supernatant was analyzed using Quantikine ELISA kits (R&D systems) for IL-2 (#D2050) and IFN-γ (#DIF50C) according to the manufacturer’s instructions. In another experiment, the MIILIPLEX

(2) Anoctamin 9 determines Ca2+ signals during activation of T-lymphocytesFrontiers in ImmunologyMarch 26, 2025Rainer Schreiber, Jiraporn Ousingsawat, Karl KunzelmannUSA). IL-2 release Cellular release of IL-2 was detected using quantikine colorimetric sandwich ELISA kit (D2050, R&D Systems, Wiesbaden-Nordenstadt, Germany). Transfected jurkat E6.1 cells were treated with PHA/PMA

(3) Mapping variant effects on anti-tumor hallmarks of primary human T cells with base-editing screensNature BiotechnologyMarch 1, 2025Zachary H. Walsh, Parin Shah, Neeharika Kothapalli, Shivem B. Shah, Gergo Nikolenyi, D. Zack Brodtman, Giuseppe Leuzzi, Meri Rogava, Michael Mu, Patricia Ho, et al.concentrations were measured with the appropriate Quantikine ELISA kit (R&D Systems, TNF, DTA00D; IL-2, D2050; IFN-γ, DIF50C) following the manufacturer’s instructions. The following supernatant dilutions were

(4) Short- and long-term efficacy of olaparib combined with chemotherapy in advanced triple-negative breast cancerAmerican journal of cancer researchJanuary 1, 2025Wang W, Yang J, Miao Y, Yao L.

(5) Ex vivo T cell differentiation in adoptive immunotherapy manufacturing: Critical process parameters and analytical technologiesBiotechnology advancesDecember 1, 2024Chen S, Nguyen TD, Lee KZ, Liu D.

(6) Influence of blood trace elements on immune responses and adverse symptoms subsequent to Sinopharm COVID-19 vaccinationScientific reportsNovember 27, 2024Abbasifard M, Kazerooni K, Taghipour Khaje Sharifi G, Bagheri-Hosseinabadi Z, Hajizadeh MR.

(7) Persistence of activated anti‐mesothelin hYP218 chimeric antigen receptor T cells in the tumour is associated with efficacy in gastric and colorectal carcinomasClinical and Translational MedicineNovember 15, 2024Sameer Mir, Abhilash Venugopalan, Jingli Zhang, Nishanth Ulhas Nair, Manjistha Sengupta, Manakamana Khanal, Chaido Stathopoulou, Qun Jiang, Raffit Hassanto 24 h of co‐culture with tumour cells were quantified using ELISA kits (R&D Systems, catalogue No. D2050, DIF50C, DTA00D). Briefly, in a 96‐well plate, 5000 cancer cells were seeded per well and then co‐incubated

(8) A Novel Monoclonal Antibody against PD-1 for the Treatment of Viral Oncogene-Induced Tumors or Other CancerCancersSeptember 1, 2024Xu X, Yan SL, Yo YT, Chiang P, Tsai CY, Lin LL, Qin A.the quantitation of IL-2 using a human IL-2 Quantikine ELISA Kit (R&D Systems, Minneapolis, MN, USA, D2050/S2050). For the nonactivated T-cell cytokine response assay, human PBMCs from healthy donors were incubated

(9) Generation of antitumor chimeric antigen receptors incorporating T cell signaling motifsScience SignalingJuly 23, 2024Lakshmi Balagopalan, Taylor Moreno, Haiying Qin, Benjamin C. Angeles, Taisuke Kondo, Jason Yi, Katherine M. McIntire, Neriah Alvinez, Sandeep Pallikkuth, Mariah E. Lee, et al.culture supernatant was harvested for the detection of cytokines by ELISA (R&D Systems, catalog no. D2050 for human IL-2 and DIF50C for human IFN-γ). Flow cytometry Cells were washed twice with PBS and stained

(10) Hainanenin-1, an oncolytic peptide, triggers immunogenic cell death via STING activation in triple-negative breast cancerCell Communication and SignalingJuly 5, 2024Xiaoxi Li, Nan Su, Haining Yu, Xiaoyan Li, Shu-lan Sunquantification of cytokines in the supernatant was performed using a human ELISA detection kit (IL-2, D2050; IL-10, D1000B, IFN-γ, DIF50C; R&D Systems, MN, USA) in accordance with the instructions provided by

(11) A Bispecific Antibody That Targets the Membrane-Proximal Region of Mesothelin and Retains High Anticancer Activity in the Presence of Shed MesothelinMolecular cancer therapeuticsJuly 1, 2024Chakraborty A, Onda M, O'Shea T, Wei J, Liu X, Bera TK, Pastan I.TNF-alpha, and IL-2 cytokine levels were measured with Quantikine ELISA kits (R&D Systems #DIF50C, #DTA00D, #D2050). Kit instructions were followed with the following modifications: the standard curves were created

(12) Serum Dehydroepiandrosterone sulfate (DHEA-S) level and its potential impact on immune responses and symptom severity after Oxford-AstraZeneca COVID-19 vaccinationInternational immunopharmacologyMay 30, 2024Abbasifard M, Dehghan Banadaki M, Taghipour Khaje Sharifi G, Rahnama A, Bagheri-Hosseinabadi Z.

(13) Dynamic immune signatures of patients with advanced non–small-cell lung cancer for infection prediction after immunotherapyFrontiers in ImmunologyJanuary 26, 2024Yung-Hung Luo, Chia-I Shen, Chi-Lu Chiang, Hsu-Ching Huang, Yuh-Min Chenenzyme-linked immunosorbent assay (ELISA) Kit (R&D Systems, Minneapolis, MN, USA; Human interleukin [IL]-2 ELISA Kit, Catalog #D2050; Human IL-6 ELISA Kit, Catalog #D6050; Human IL-10 ELISA Kit, Catalog #D1000B; Human tumor necrosis

(14) BCMA- and CST6-specific CAR T cells lyse multiple myeloma cells and suppress murine osteolytic lesionsJournal of Clinical InvestigationJanuary 2, 2024Fumou Sun, Yan Cheng, Jin-Ran Chen, Visanu Wanchai, David E. Mery, Hongwei Xu, Dongzheng Gai, Samer Al Hadidi, Carolina Schinke, Sharmilan Thanendrarajan, et al.FN50, no. 310906), and IFN-γ and IL-2 cytokines were measured using ELISA kits (R&D Systems, DIF50C and D2050) and were produced by activated CAR-T cells in supernatants of CAR-T and MM cell cocultures after 24

(15) In the Search for Biomarkers of Pulmonary Arterial Hypertension, Are Cytokines IL-2, IL-4, IL-6, IL-10, and IFN-Gamma the Right Indicators to Use?International journal of molecular sciencesSeptember 5, 2023Tomaszewski M, Mertowska P, Janczewska M, Styczeń A, Mertowski S, Jonas K, Grywalska E, Kopeć G.

(16) Possible Correlation between Urocortin 1 (Ucn1) and Immune Parameters in Patients with EndometriosisInternational journal of molecular sciencesApril 24, 2023Abramiuk M, Frankowska K, Kułak K, Tarkowski R, Mertowska P, Mertowski S, Grywalska E.

(17) Novel scFv against Notch Ligand JAG1 Suitable for Development of Cell Therapies toward JAG1-Positive TumorsBiomoleculesMarch 2, 2023Silva G, Rodrigues AF, Ferreira S, Matos C, Eleutério RP, Marques G, Kucheryava K, Lemos AR, Sousa PMF, Castro R, et al.with HepG2/Ctrko (JAG1+) cells from the activation assays using Quantikine ELISA kits (R&D Systems, D2050 and DIF50C) following the manufacturer’s instructions. 2.8. Western Blotting and Flow Cytometry Both

(18) Point-of-Care Platform for Diagnosis of Venous Thrombosis by Simultaneous Detection of Thrombin Generation and D-Dimer in Human PlasmaAnalytical ChemistryJanuary 17, 2023Chunxiao Hu, Valerio F. Annese, Michael P. Barrett, David R. S. Cummingcoagulation factor III/TF (2339-PA) was purchased from R&D Systems. The TECO D-dimer agglutination kit (D2050-000) was purchased from Alpha Laboratories Ltd. It includes one vial containing 1.7 mL of suspension

(19) Interleukine-2 serum level in breast cancer patientsCaspian J Intern MedJanuary 1, 2023Tutik Harjianti, Andi Fachruddin, Rahmawati Minhajat, Sahyuddin Saleh, Dimas Bayureagent kit used for human IL-2 examination is a product R&D system, Minneapolis, MN 55413, USA, Cat: D2050, Lot: 298415, ED: 21 Nov 2012. The differences of IL-2 level are analyzed between breast cancer patients

(20) MET-Induced CD73 Restrains STING-Mediated Immunogenicity of EGFR-Mutant Lung CancerCancer researchNovember 2, 2022Yoshida R, Saigi M, Tani T, Springer BF, Shibata H, Kitajima S, Mahadevan NR, Campisi M, Kim W, Kobayashi Y, et al.no. DIP100), human IFNγ (R&D Systems, catalog no. DIF50C), and human IL2 (R&D Systems, catalog no. D2050) ELISAs were performed according to the manufacturer's instructions. Conditioned media from each cell

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IL-2 ELISA

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IL-2 ELISA

IL-2 ELISA

Human IL-2 Quantikine ELISA Kit from R&D Systems, a Bio-Techne Brand

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R&D Systems, a Bio-Techne Brand

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Human IL-2 Quantikine ELISA Kit from R&D Systems, a Bio-Techne Brand

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