Human FGF basic/FGF2/bFGF Quantikine ELISA Kit DFB50 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemHuman FGF basic/FGF2/bFGF Quantikine ELISA Kit
CompanyR&D Systems, a Bio-Techne Brand
Price $680.00Supplier Page View Company Product Page
Catalog NumberDFB50
Quantity1 Kit (also 1 Pack (50 Plates), 1 Pack (6 Plates))
ApplicationsELISA
Detection Range10.00 - 640 pg/mL
ReactivityHuman
Sample TypeCell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL)
Sensitivity3 pg/mL
Detection TargetFGF basic/FGF2/bFGF
Assay Time4.5 hours
StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date
NCBI Full Gene Namefibroblast growth factor 2
NCBI Gene AliasesBFGF, FGF-2, FGFB, HBGF-2

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Doxorubicin enhances adipogenesis in an FGF2-dependent manner and induces a tumour-promoting secretory phenotypeJournal of Bone OncologyApril 1, 2026Lauren M. Kreps, Edward Yakubovich, Huijun Zhao, Selam Yimer, Edward Yang, Juliet Cruz, Barbara Vanderhyden, Christina L. Addisonconcentration of FGF2 in conditioned supernatants was performed using the Quantikine ELISA kit (cat #DFB50, R&D Systems, Minneapolis, MN), according to manufacturer’s instructions. Absorbance was measured using

(2) Implantation of human mesenchymal stromal cell (MSC)-pellet for therapeutic angiogenesisCytotherapyFebruary 1, 2026Akiko Mammoto, Priscilla Kyi, Mikaela Scheer, Tadanori Mammotoand FGF2 proteins in mouse plasma were measured using Quantikine® ELISA kits (Bio-Techne DFA00B and DFB50, respectively) according to the manufacturer’s instructions. Mouse plasma samples were diluted with

(3) FGF-2–Overexpressing Adipose-Derived Stem Cells as a Paracrine Platform for Angiogenesis-Driven Tissue RegenerationCellular and Molecular BioengineeringDecember 29, 2025Daisuke Seki, Michiyo Hondausing the Quantikine® Human FGF Basic Immunoassay (R&D Systems, Inc., Minneapolis, MN, USA; Cat. No. DFB50) and the Human VEGF ELISA Kit (Proteintech Group, Inc., Rosemont, IL, USA; Cat. No. KE00085), respectively,

(4) Safety and efficacy of long-shelf-life allogeneic adipose-derived mesenchymal stem cell line-derived platelet-like cells for refractory foot ulcers: A translational preclinical and phase 1/2a studyRegenerative therapyDecember 1, 2025Obara H, Matsubara K, Fujimura N, Matsubara Y, Ono-Uruga Y, Yazawa M, Okui J, Sato Y, Kitagawa Y.

(5) Hepatocyte Growth Factor-Mediated Chondrocyte Proliferation Induced by Adipose-Derived MSCs from Osteoarthritis Patients and Its Synergistic Enhancement by Hyaluronic AcidInternational Journal of Molecular SciencesSeptember 23, 2025Samuel Jaeyoon Won, Hyun-Joo Lee, Dae-Yong Kim, Hyeonjeong Noh, Song yi Lee, Ji Ae Yoo, Yoon Sang Jeon, Heebeom Shin, Dong Jin Ryuand analyzed using ELISA kits specific for human HGF (R&D Systems, DHG00B), TGF-β1 (DB100C), FGF2 (DFB50), and VEGF (DVE00). All samples were measured in duplicate, and concentrations were normalized to pg/mL.

(6) Autocrine Fibroblast Growth Factor Receptor 1 Signaling Activates Lactate Dehydrogenase A‐Aerobic Glycolysis for Advanced Human Prostate Tumor GrowthProstate CancerSeptember 16, 2025Xiaoming Xu, Li Wang, Huafeng Pan, Tingitng Gu, Zhongliang Cheng, Tianjun Peng, Jianting Zhang, Jiaren Panof FGF2 in the supernatants were determined using human FGF basic ELISA Quantikine assay kit (Cat#: DFB50, R&D Systems, Inc., Minneapolis, MN, USA) according to the manufacturer's instruction. Data were expressed

(7) Extracellular vesicles derived from clonal mesenchymal stromal cells preconditioned by indirect hypoxia modulate immune responses in diabetic mice more effectively than directly preconditioned vesiclesStem Cell Res TherAugust 26, 2025Mehdi Soleymani-Goloujeh, Mahnaz Babaahmadi, Faezeh Shekari, Mojgan Barati, Nasrin Fallah, Mahmoud Alipour Choshali, Nima Makvnd Gholipour, Maryam Hezavehei, Masoumeh Azimi, Saeed Yakhkeshi, et al.R&D Systems, USA), and the amounts of HGF and bFGF were measured via human Quantikine® ELISA kits (DHG00 and DFB50, R&D Systems, USA) according to the manufacturer’s instructions. For the in vivo functional assay, the

(8) (+)-a-Longipinene alleviates murine laser-induced choroidal neovascularization: Evidence from in vivo and in vitro experimentsInternational immunopharmacologyJune 26, 2025Zhu M, Wu M, Yang A, Tu Y, Sun C, Na Y, Zhang Y, Wang Y, Su H, Liu X.

(9) Deficiency of fibroblast growth factor 2 promotes contractile phenotype of pericytes in ascending thoracic aortic aneurysmAmerican Journal of Physiology-Heart and Circulatory PhysiologyMay 1, 2025Weijian Huang, Jennifer C. Hill, Sakshi Patel, Tara D. Richards, Ibrahim Sultan, David J. Kaczorowski, Julie A. Phillippiwere used to determined FGF2 levels in adventitia via Human FGF2 Quantikine ELISA Kit (R&D Systems, DFB50) following the manufacturer’s instructions. Statistical analyses All statistical analyses were performed

(10) Basal cell adhesion molecule (BCAM) promotes mesothelial-to-mesenchymal transition and tumor angiogenesis through paracrine signalingCell communication and signaling : CCSMarch 13, 2025Sivakumar S, Lieber S, Dietze R, Beutgen VM, Sutor EC, Heidemann S, Finkernagel F, Teply-Szymanski J, Nist A, Stiewe T, et al.enzyme-linked immunosorbent assay (ELISA), using the following kits: AREG (Cat. #DAR00, R&D Systems/Bio-Techne), FGF2 (Cat. #DFB50, R&D Systems/Bio-Techne), VEGF-A (Cat. #BMS277-2, Life/Thermo Fisher), VEGF-B (Cat. #CSB-E04758H-96,

(11) Increasing the concentration of plasma molecules improves the biological activity of platelet-rich plasma for tissue regenerationScientific reportsFebruary 6, 2025Sánchez M, Mercader Ruiz J, Marijuán Pinel D, Sánchez P, Fiz N, Guadilla J, Azofra J, Beitia M, Delgado D.Bio-techne), HGF (DHG00B; Bio-techne), PDGF-AB (DHD00C; Bio-techne), EGF (DEG00; Bio-techne); FGF (DFB50; Bio-techne), BDNF (DBD00; Bio-techne) and α2M (ab108888; Abcam, Cambridge, UK). Technical duplicates

(12) Comparison of bioactive substances in novel-developed freeze-dried platelet-rich plasma (PRP) and activated normal PRP, and investigation of bioactive substance levels after long-term storageRegenerative TherapyDecember 1, 2024Ryosuke Nakajima, Yoshitomo Saita, Yohei Kobayashi, Takanori Wakayama, Sayuri Uchino, Yasumasa Momoi, Nanako Yamamoto, Muneaki IshijimaUSA) were used to measure the concentrations of growth factors and cytokines: TGF-β (DB100B), bFGF (DFB50), EGF (DEG00), TIMP-1 (DTM100), IL-1ra (DRA00B), IL-6 (D6050), and MMP-9 (DMP900). All procedures were

(13) Co-delivery of Liposomal Ketoconazole and Bevacizumab for Synergistical Inhibition of Angiogenesis Against Endometrial CancerMolecular BiotechnologySeptember 4, 2024Shanshan Wang, Jinglin Miao, Ping Zhu, Li XuVEGF Quantikine ELISA KitCatalog #: DVE00, Human FGF basic/FGF2/bFGF Quantikine ELISA Kit Catalog #: DFB50, R&D systems, Minneapolis, USA). Co-culture Assay of Cancer Cells with Fibroblasts The Trans-well

(14) Amelioration of Photoreceptor Degeneration by Intravitreal Transplantation of Retinal Progenitor Cells in RatsInternational journal of molecular sciencesJuly 24, 2024Yang J, Lewis GP, Hsiang CH, Menges S, Luna G, Cho W, Turovets N, Fisher SK, Klassen H.

(15) Development of composite functional tissue sheets using hiPSC-CMs and hADSCs to improve the cardiac function after myocardial infarctionBioactive materialsJuly 1, 2024Zhang J, Li J, Qu X, Liu Y, Sun L, Harada A, Hua Y, Sougawa N, Tabata A, Liu L, et al.factor (VEGF, DVE00), hepatocyte growth factor (HGF, DHG00B) and basic fibroblast growth factor (β-FGF, DFB50, R&D Systems, Minneapolis, MN, USA, Quantikine ELISA), and was performed according to the manufacturer’s

(16) Osteogenic effects and safety of human induced pluripotent stem cell-derived megakaryocytes and platelets produced on a clinical scaleRegenerative therapyJune 1, 2024Arai T, Shiga Y, Mukai M, Takayama N, Tashiro S, Tajiri I, Kosaka K, Sato M, Nakamura S, Okamoto H, et al.

(17) L-PRF Secretome from Both Smokers/Nonsmokers Stimulates Angiogenesis and Osteoblast Differentiation In VitroBiomedicinesApril 16, 2024Ríos S, González LG, Saez CG, Smith PC, Escobar LM, Martínez CE.

(18) A Comparative Analysis of Innate Immune Responses and the Structural Characterization of Spike from SARS-CoV-2 Gamma Variants and SubvariantsMicroorganismsApril 2, 2024Scovino AM, Dahab EC, Diniz-Lima I, de Senna Silveira E, Barroso SPC, Cardoso KM, Nico D, Makhoul GJ, da Silva-Junior EB, Freire-de-Lima CG, et al.

(19) Fluoride-Incorporated Apatite Coating on Collagen Sponge as a Carrier for Basic Fibroblast Growth FactorInternational journal of molecular sciencesJanuary 25, 2024Pal A, Oyane A, Nakamura M, Koga K, Nishida E, Miyaji H.

(20) Expression of HLA-DR by mesenchymal stromal cells in the platelet lysate era: an obsolete release criterion for MSCs?Journal of translational medicineJanuary 9, 2024Kuçi Z, Piede N, Vogelsang K, Pfeffermann LM, Wehner S, Salzmann-Manrique E, Stais M, Kreyenberg H, Bonig H, Bader P, et al.ELISA according to the manufacturer’s instructions (human FGF basic/FGF2, Quantikine Elisa, Cat. No DFB50, R&D Systems, Wiesbaden, Germany) by using a microplate reader, Victor③multilabel reader and Wallac

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FGF basic/FGF2/bFGF ELISA

Human FGF basic/FGF2/bFGF Quantikine ELISA Kit

FGF basic/FGF2/bFGF ELISA

Human FGF basic/FGF2/bFGF Quantikine ELISA Kit from R&D Systems, a Bio-Techne Brand

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R&D Systems, a Bio-Techne Brand

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Human FGF basic/FGF2/bFGF Quantikine ELISA Kit from R&D Systems, a Bio-Techne Brand

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