8-Isoprostane ELISA Kit 516351 from Cayman Chemical

Product Specs
Item8-Isoprostane ELISA Kit
CompanyCayman Chemical
Price $1,506.00Supplier Page View Company Product Page
Catalog Number516351
Quantity480 Well
ApplicationsELISA
Detection Range0.8-500 pg/ml
DetectionColorimetric
MethodEIA
Sensitivity3 pg/ml
Research AreaEnvironmental Toxicology, Lipid Biochemistry, Lipid Peroxidation, Oxidative Stress & Reactive Species, Reactive O2/N2 Pathways, Toxicology & Xenobiotic Metabolism
Storage-20
OriginUSA
Source/Expression SystemAnimal/Bovine:Animal/Eel:Animal/Mouse:Animal/Rabbit

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Cayman Chemical

Cayman Chemical Company 1180 East Ellsworth Road

Ann Arbor, Michigan 48108

United States

Phone: (800) 364-9897
(734) 971-3640

Company Profile

Website: http://www.caymanchem.com/

(1) SOD1 deficiency drives ferroptosis-linked oxidative and reproductive aging, mitigated by ginseng root extractGeroScienceJanuary 12, 2026Juewon Kim, Shuichi Shibuya, Yusuke Ozawa, Yorino Sato, Kazuhiro Kawamura, Takahiko Shimizuin the plasma and skin homogenate were determined using the 8-isoprostane enzyme immunoassay kit (#516351; Cayman Chemical Company, MI, USA) in accordance with the manufacturer’s protocol. Protein content in

(2) Peripheral biomarkers associated with apathy and predicting response to methylphenidate: Secondary analysis of the Apathy in Dementia Methylphenidate Trial 2 (ADMET2) studyInternational PsychogeriatricsJanuary 10, 2026Krushnaa Sankhe, Myuri Ruthirakuhan, Ana C. Andreazza, Olga Brawman-Mintzer, Suzanne Craft, Nathan Herrmann, Zahinoor Ismail, Alan J. Lerner, Allan I. Levey, Jacobo Mintzer, et al.oxidative stress was measured by 4-hydroxy-nonenal (4-HNE) and 8-isoprostane (8-ISO, Cayman Chemical; Item #516351) with standard ELISA. Biomarkers with unreadable results, insufficient volume, or absence of signal

(3) Low Dose GLP-1 Therapy Attenuates Pathological Cardiac and Hepatic Remodelling in HFpEF Independent of Weight LossbioRxivOctober 2, 2025Mahmoud H. Elbatreek, Zhen Li, Xiaoman Yu, Natalie D. Gehred, Tatiana Gromova, Jingshu Chen, Naoto Muraoka, Martin Jensen, Vinay Kartha, Chris Carrico, et al.cholesterol levels were measured using kit # MAK043 (Millipore Sigma, USA). Plasma levels of 8-isoprostane (# 516351, Cayman Chemical, USA), and hs-CRP (# EKF57936, Biomatik, Canada) were determined using commercially

(4) Hydrogen Sulfide Deficiency and Therapeutic Targeting in Cardiometabolic HFpEF: Evidence for Synergistic Benefit With GLP-1/Glucagon AgonismJACC. Basic to translational scienceOctober 1, 2025Doiron JE, Elbatreek MH, Xia H, Yu X, Gehred ND, Gromova T, Chen J, Driver IH, Muraoka N, Jensen M, et al.according to manufacturer’s protocols. Enzyme-linked immunosorbent assay Plasma 8-isoprostane (# 516351 Cayman Chemical, USA) and 3-nitrotyrosine (# NBP2-66363, Novus Biologicals) levels were measured by

(5) Placental Morphology and Metabolomic Profile in Uncomplicated Metabolically Healthy Obese PregnancyBiomedicinesSeptember 3, 2025Ousseynou Sarr, Akasham Rajagopaul, Shuang Zhao, Xiaohang Wang, David Grynspan, Genevieve Eastabrook, Liang Li, Timothy R. H. Regnault, Barbra de Vrijerplacental tissues were measured using the 8-isoprostane enzyme-linked immunosorbent assay kit (kit no 516351, Cayman Chemical), following the manufacturer’s instructions. Briefly, 50 mg placenta samples were homogenized

(6) Urinary prostaglandin metabolites as biomarkers for human labour: Insights into future predictorsPLoS OneJuly 14, 2025Eilidh M. Wood, Kylie K. Hornaday, Matthew Newton, Melinda Wang, Stephen L. Wood, Donna M. Slatermeasured by ELISA kit (Cayman Chemical, Ann Arbor, MI, item numbers #516671, #501001, #501100, and #516351, respectively). Prostaglandin E2 metabolite (PGEM) was measured using a PGE Metabolite ELISA kit (Cayman Chemical

(7) Redox Imbalance Is Associated with Neuronal Apoptosis in the Cortex of Neonates Gestated Under Chronic HypoxiaAntioxidants (Basel)June 15, 2025Esteban G. Figueroa, Rodrigo L. Castillo, Adolfo A. Paz, Matías Monsalves-Alvarez, Francisca Salas-Pérez, Ximena Calle, Tamara A. Jiménez, Emilio A. Herrera, Alejandro Gonzaléz-Candia(Colorimetric) (ab239709, Cayman Chemical Company, Ann Arbor, MI, USA) and 8-Isoprostane ELISA Kit (516351, Cayman Chemical Company, Ann Arbor, MI, USA), according to the manufacturers’ guidelines. As previously

(8) Impact of Chronic Cold Water Immersion and Vitamin D3 Supplementation on the Hippocampal Metabolism and Oxidative Stress in RatsCellsApril 26, 2025Daria Korewo-Labelle, Mateusz Jakub Karnia, Dorota Myślińska, Jan Jacek KaczorDetermination of 8-Isoprostanes (8-iso) Levels An enzymatic immunoassay ELISA analyzing 8-isoprostanes (516351; Cayman Chemical; Ann Arbor, MI, USA) in the hippocampal lysate was used to assess free radical-induced

(9) Systemic Responses Towards Oxy-Inflammation, Hormones, and Mood in Breast Cancer Survivors: Preliminary Evidences from Dragon Boat Endurance RaceJournal of clinical medicineApril 7, 2025Montorsi M, Vezzoli A, Mrakic Sposta F, Gussoni M, Brizzolari A, Bosco G, Dellanoce C, Barassi A, Picconi B, Ranuncoli C, et al.measuring 8-isoprostane concentration (8-iso-PGF2α) (Cayman Chemical, Ann Arbor, Michigan, USA, Item No. 516351). The method was previously described [19,22]. DNA damage (urinary concentrations of 8-OH-dG) was measured

(10) Prothrombotic State and Vascular Damage in Angiotensin II–Induced Hypertension: Influence of Waterpipe Smoke ExposureOxidative Medicine and Cellular LongevityJanuary 23, 2025Sumaya Beegam, Suhail Al-Salam, Nur Elena Zaaba, Ozaz Elzaki, Abderrahim Nemmar8-Isoprostane concentration and catalase activity (kits from Cayman Chemicals, Michigan, USA; Catalog No. 516351 and 707002) were measured according to the vendor's protocol. The quantification of nitric oxide (NO)

(11) Interactive effects of high n-3 PUFA intake and cyclic heat stress under two dietary antioxidant levels in broiler chickensFrontiers in physiologyJanuary 1, 2025Rezar V, Pal MP, Levart A, Nemec Svete A, Pirman T, Salobir J, Leskovec J.501320, Cayman Chemical, Ann Arbor, MI, United States) and plasma F2-isoprostanes (iPF2α- III) (No. 516351, Cayman Chemical, Ann Arbor, MI, United States) and Hsp70 (Wuhan Fine Biotech Co. (No. ECH00723)). The

(12) Evaluation of Ocular and Systemic Oxidative Stress Markers in Patients with Diabetic RetinopathyLife (Basel)December 2, 2024Ana Karen López-Contreras, Diana Esperanza Arévalo-Simental, Fermín Paúl Pacheco-Moisés, María Guadalupe Martínez-Ruíz, Cecilia Olvera-Montaño, Ricardo Raúl Robles-Rivera, Sonia Sifuentes-Franco, Tannia Isabel Campos-Bayardo, Selene Guadalupe Huerta-Olvera, Adolfo Daniel Rodríguez-Carrizalezaccording to the methodology described by the kit’s manufacturer (8-isoprostanes ELISA kit, Item No. 516351, Cayman chemicals, Ann Arbor, MI, USA). This assay is based on the competition between 8-isoprostanes

(13) Diet Biofunctionality in Modulating Cardiovascular Parameters in Obese Patients After Bioenteric Intragastric Balloon Bariatric SurgeryNutrientsNovember 26, 2024Edyta Barbara Balejko, Jarosław Lichotaaccuracy of ±1% ± 0.010 absorbance and a measurement range of 0.156–10 ng/mL;-Isoprostane (catalogue no. 516351, Cayman Chemical Company, Ann Arbor, MI, USA), with a method accuracy of ±1% ± 0.010 absorbance and

(14) Randomized Trial of Dietary Acid Reduction and Acid-Base Status of Patients With CKD and Normal Estimated GFRKidney International ReportsNovember 9, 2024Nimrit Goraya, Nicolaos E. Madias, Jan Simoni, Maninder Kahlon, Nazan Aksan, Donald E. Wessonthe ethyl acetate phase,58 and screened by enzyme-linked immunosorbent assay method (Procedure No. 516351, Cayman Chemical, Ann Arbor, MI). Urine levels of N-acetyl-β-D-glucosamine excretion, angiotensinogen

(15) Intrauterine growth-restricted pregnant rats, from placental ischemic dams, display preeclamptic-like symptoms: A new rat model of preeclampsiaPhysiological reportsNovember 1, 2024Smith J, Powell M, Cromartie W, Smith S, Jones K, Castillo A, Shaw J, Editone J 3rd, Howard A, Tatum R, et al.8‐isoprostane, markers of ROS via phospholipid oxidation, were measured by an 8‐Isoprostane ELISA Kit (516351, Cayman Chemical, Ann Arbor, MI). 8‐isoprostanes amounts were calculated in accordance with the manufacturer's

(16) Oxidative Stress in Polycystic Ovary Syndrome: Impact of Combined Oral ContraceptivesAntioxidants (Basel)September 26, 2024Nicolás Santander, Esteban G. Figueroa, Alejandro González-Candia, Manuel Maliqueo, Bárbara Echiburú, Nicolás Crisosto, Francisca Salas-Pérezassay kit (ab239709, Abcam, Cambridge, UK) and the 8-isoprostane concentration was measured by ELISA (516351, Cayman Chemical Company, Ann Arbor, MI, USA) following the manufacturers’ guidelines. 2.7. Ferric

(17) Blockade of thromboxane A2 signaling attenuates ethanol‐induced myocardial inflammatory response in miceAlcohol, Clinical and Experimental ResearchAugust 1, 2024Weilun Ai, Carol A. Casey, Paras Kumar Mishra, Yazen Alnouti, Sohel Daria, Viswanathan Saraswathimetabolite of TXA2, and 8-iso were measured by the TXB2 ELISA kit (No. 501020) and 8-iso ELISA kit (No. 516351), respectively (Cayman Chemical, Ann Arbor, MI, USA). Mouse plasma samples were diluted 1:10 as per

(18) Cannabidiol effectively prevents oxidative stress and stabilizes hypoxia-inducible factor-1 alpha (HIF-1α) in an animal model of global hypoxiaScientific ReportsJuly 10, 2024Hanna Kletkiewicz, Michał S. Wojciechowski, Justyna RogalskaPGF2α content was determined with an 8-Isoprostane ELISA Kit (Cayman Chemicals, USA, Catalogue number: 516351). The samples were homogenized using an ultrasonic homogenizer (Sonics Vibra-Cell; Sonics & Materials,

(19) Preliminary Study on the Restoration of the Phospholipid Profile in Serum from Patients with COVID-19 by Treatment with Vitamin ECurrent Issues in Molecular BiologyJuly 8, 2024María Elena Soto, Linaloe Manzano-Pech, Verónica Guarner-Lans, Adrían Palacios-Chavarría, Rafael Ricardo Valdez-Vázquez, Raúl Martínez-Memije, Mohammed El-Hafidi, Félix Leao Rodríguez-Fierros, Israel Pérez-TorresDeterminations A total of 50 μL of serum were used for these determinations. The quantification of 8-isoprostane (#516351 ELISA Kit), thromboxane B2 (TXB2) (#501020 ELISA Kit), PGE2 (#514531 ELISA Kit), and 6-keto-PGF1α (#515211

(20) Dietary Supplementation with n-3 Polyunsaturated Fatty Acids Delays the Phenotypic Manifestation of Krabbe Disease and Partially Restores Lipid Mediator Production in the Brain—Study in a Mouse Model of the DiseaseInternational Journal of Molecular SciencesJune 28, 2024Cinzia Signorini, Giovanna Pannuzzo, Adriana Carol Eleonora Graziano, Elena Moretti, Giulia Collodel, Venera Cardilemethanol (5 mL). Eight isoprostanes were immunologically detected by a competitive immunoassay (Item No. 516351, Cayman Chemical, Ann Arbor, MI, U.S.A.). A reference curve was applied to determine 8-IsoP levels in

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8-Isoprostane ELISA Kit

8-Isoprostane ELISA Kit

8-Isoprostane ELISA Kit from Cayman Chemical

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Cayman Chemical

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8-Isoprostane ELISA Kit from Cayman Chemical

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