Fig 1: Analysis of CPF action on p-P38α (A), P75NTR (B), and PAI-1 (C) levels. Results are shown as a percentage of control values. Results of protein content were normalized by total protein concentrations. *** p ≤ 0.001 compared to control. &&& p ≤ 0.001 compared to P38α-silenced cells exposed to CPF.
Fig 2: PAI-1, MMP-9, and P75NTR silencing effect on SN56 cell viability and gene expression. Control: SN56 cells transfected without siRNA. Negative (Neg.) control: SN56 cells transfected with control siRNA. PAI-1-siRNA: transfected with siRNA against PAI-1. MMP9-siRNA: transfected with siRNA against MMP-9. P75NTR-siRNA: transfected with siRNA against P75NTR. MTT analysis shows that PAI-1, MMP-9, and P75NTR knockout did not significantly induce cell damage after 48 h (A). PAI-1, MMP-9, and P75NTR downregulation could be detected by RT-PCR analysis 48 h after transfection (B,C). Values are given as mean ± SEM of three separate experiments from cells of different cultures, each one performed in triplicate. *** p ≤ 0.001 compared to the control.
Fig 3: PFOS (0.1–40 µM) effects on (A) proNGF and (C) mNGF protein content after one- and fourteen-days of treatment. Effects of PAI-1 knockdown, treatment with PFOS (10 µM) or ruPA (15 µM), ruPA treatment of PAI-1 silenced cells or PFOS co-treatment with ruPA of wildtype cells or PFOS co-treatment with ruPA of PAI-1 silenced cells on proNGF protein content (B). Effects of PAI-1 or MMP-9 knockdown, treatment with PFOS (10 µM) or ruPA (15 µM), ruPA treatment of simultaneous PAI-1 and MMP-9 silenced cells, or PFOS co-treatment with ruPA of wildtype cells or PFOS co-treatment with ruPA of single or simultaneous PAI-1 and/or MMP-9 silenced cells on mNGF protein content (D). Data represent the mean ± SEM of three separate experiments from cells of different cultures, each one performed in triplicate. *** p < 0.001 compared to the control. ### p ≤ 0.001 compared to the PFOS treatment. &&& p ≤ 0.001 compared to the PFOS treatment. δδδ p ≤ 0.001 compared to the ruPA co-treated cells with PFOS. ιιι p ≤0.001 compared to the PFOS treatment of MMP-9-silenced cells.
Fig 4: PFOS (0.1–40 µM) effects on (A) uPA, (B) PAI-1, and (C) MMP-9 levels in SN56 cell homogenates after one- and fourteen-days of treatment. The mean ± SEM was obtained from data of three replicates of cultures performed three different times. *** p ≤ 0.001, significantly different from the controls. &&& p ≤ 0.001 compared to the PFOS treatment.
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