Fig 1: Level of plasma RARRES2 (A), ADIPOQ (B) and NAMPT(C) concentrations of broiler hens fed with different concentrations of GSE dietary supplementation.At 33rd week, plasma adipokines concentration was assessed using specific ELISA assay in 11 animals from each group of animals. A: animals fed with control diet without GSE supplementation, B and C: animals supplemented with GSE at 0.5% and 1% of the total diet composition, respectively, starting at 4 week-old until 40 week-old, and D: supplementation at 1% of the total diet composition starting at hatch until 40 week-old. Results are presented as lsmeans ± S.E.M. P values and different individual letters indicate a significant effect of the diet between A, B and C groups (experiment 1) or between A and D groups (experiment 2). P value was considered as significant if P < 0.05. **** P < 0.0001.
Fig 2: Plasma concentration of RARRES2 (A), ADIPOQ (B) and NAMPT (C) in broiler hens fed with different concentrations of GSE dietary supplementation or with a control diet.A: animals fed with control diet without GSE supplementation (n = 92), B and C: animals supplemented with GSE at 0.5% and 1% of the total diet composition, respectively, starting at 4 week-old until 40 week-old (n = 80), and D: supplementation at 1% of the total diet composition starting at hatch until 40 week-old (n = 72). The starting period corresponds to the hatching until the 4th week, the growing period corresponds to the 4th week until 17th week, the « before laying » period corresponds to the 18th week to the 23rd week and the laying period corresponds to the 24th week until the 40th week. Results are presented as lsmeans ± SEM. P values of the effects of the stage when the supplementation is applied (TimeSuppl) and diet were considered as significant if P < 0.05. Different individual letters (a, b and c) indicate a significant effect of the diet. **** P < 0.0001 (diet effect).
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