Description
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Sulfamethoxazole antigen. Standards or samples are added to the appropriate microtiter plate wells with Sulfamethoxazole specific antibody and Horseradish Peroxidase (HRP) conjugated anti-antibody. The competitive inhibition reaction is launched between pre-coated Sulfamethoxazole and Sulfamethoxazole in standards or samples with the Sulfamethoxazole special antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Sulfamethoxazole in the standards or samples. The color development is stopped and the intensity of the color is measured