Description
Intended Uses: This mouse IL-17A antigen assay is intended for the quantitative determination of total mouse IL-17A antigen in cell culture media. For research use only.
Principle of the Assay: Mouse IL-17A will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, biotinylated primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with horseradish peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of mouse IL-17A. The amount of color development is proportional to the concentration of mouse IL-17A in the samples.
Background: Mouse Interleukin-17A (aka IL-17, IL-17A or CTLA-8) is a 133 amino acid disulfide-linked homodimeric glycoprotein that is the founding member of the IL-17 family of proteins [1-2]. IL-17A is a proinflammatory cytokine that participates in neutrophil recruitment and is primarily expressed in CD4+ T cells [3]. IL-17A has been shown in a mouse knockout model to play a vital role in allergen-specific immune responses via T cell activation [4]. It binds to the IL-17RA and IL-17RC receptors which are expressed in TH17, CD8+ T, gammadeltaT, NK, NKT and LTi cells [5]