PathScan ® Total Akt1 Sandwich ELISA Kit from Cell Signaling Technology

CST's PathScan® Total Akt1 Sandwich ELISA Kit detects endogenous levels of Akt1 including phosphorylated and nonphosphorylated. Inhibition of phosphorylation of Akt at serine 473 by LY294002 in NIH/3T3 cells is detected by PathScan Phospho-Akt1 (Ser473) Sandwich ELISA kit, #7160, while the level of total Akt1 protein detected by this ELISA kit #7170 is unchanged, as seen in Figure 1. Similar results have also observed in Jurkat cells (data not shown). This ELISA kit does not detect related kinases, such as PKC and p70 S6 kinase. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species