PathScan ® Phospho-Akt1 (Ser473) Sandwich ELISA Kit 7160C from Cell Signaling Technology

Product Specs
ItemPathScan ® Phospho-Akt1 (Ser473) Sandwich ELISA Kit
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number7160C
Quantity1 Kit
ApplicationsELISA
Detection TargetAkt1 (Ser473) phosphate
ModificationsAkt1 (Ser473) phosphate
NCBI Full Gene NameAKT serine/threonine kinase 1
NCBI Gene AliasesAKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
Research AreaPI3K / Akt Signaling
ReactivityH, M, R

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(1) Co‐targeting FAK and Gli1 inhibits the tumor‐associated macrophages‐released CCL22‐mediated esophageal squamous cell carcinoma malignancyMedComm (2020)December 1, 2023Jie Chen, Yanmeng Zhu, Di Zhao, Lingyuan Zhang, Jing Zhang, Yuanfan Xiao, Qingnan Wu, Yan Wang, Qimin Zhanin indicated ESCC cells, PathScan phospho‐AKT (Thr308; Cat# 7252; CST) and phospho‐AKT (Ser473; Cat# 7160; CST) sandwich ELISA kits were used to evaluate the regulatory effect of several AKT mutants with/without

(2) HER2-Selective and Reversible Tyrosine Kinase Inhibitor Tucatinib Potentiates the Activity of T-DM1 in Preclinical Models of HER2-positive Breast CancerCancer Research CommunicationsSeptember 25, 2023Devra Olson, Janelle Taylor, Kelsi Willis, Kelly Hensley, Sean Allred, Margo Zaval, Lauren Farr, Robert Thurman, Nishi Jain, Renee Hein, et al.Phosphorylation ELISAs Phosphorylation of HER2 (pan-Tyr, #7968), HER3 (pan-Tyr, #7911), AKT (phos-AKT1, #7160C), and MEK (phos-MEK1/2, #7175C; all from CST) was determined using ELISAs. BT-474 cells (1.5 × 105 cells

(3) Effect of Exogenous Hydrogen Sulfide and Polysulfide Donors on Insulin Sensitivity of the Adipose TissueBiomoleculesApril 28, 2022Jolanta Kowalczyk-Bołtuć, Krzysztof Wiórkowski, Jerzy BełtowskiPathScan Total Akt1 Sandwich ELISA Kit (Kit #7170) and PathScan Phospho-Akt1 (Ser473) Sandwich ELISA Kit (#7160), respectively. Adipose tissue slices were homogenized in 10 mM Tris (pH 7.4) containing 100 mM NaCl,

(4) Targeted mutagenesis on PDGFRα-Fc identifies amino acid modifications that allow efficient inhibition of HCMV infection while abolishing PDGF sequestrationPLOS PathogensMarch 29, 2021Svenja Feldmann, Immanuel Grimm, Dagmar Stöhr, Chiara Antonini, Peter Lischka, Christian Sinzger, Cora StegmannAkt phosphorylation by ELISA. The PathScan Phospho-Akt1 (Ser473) Sandwich ELISA Kit (Cell Signaling #7160) was used for detection of Akt phosphorylation according to the manufacturer’s instructions. In short,

(5) Expression of UPR effector proteins ATF6 and XBP1 reduce colorectal cancer cell proliferation and stemness by activating PERK signalingCell Death & DiseaseJuly 1, 2019Claudia N. Spaan, Wouter L. Smit, Jooske F. van Lidth de Jeude, Bartolomeus J. Meijer, Vanesa Muncan, Gijs R. van den Brink, Jarom Heijmansanti-phospho-eIF2α (Cell Signaling #3398, 1:1000), anti-CHOP (Cell Signaling #2895, 1:1000), anti-XBP1 (Santa Cruz 7160, 1:500), Anti-eIF2α (Cell Signaling #2103, 1:1000), anti-c-Myc (Santa Cruz #764, 1:1000), anti-ATF6

(6) Olaquindox disrupts tight junction integrity and cytoskeleton architecture in mouse Sertoli cellsOncotargetOctober 24, 2017Wu D, Huang CJ, Jiao XF, Ding ZM, Zhang JY, Chen F, Wang YS, Li X, Huo LJ.Affinity",1:500, Phospho-mTOR antibody,"DF3308, Affinity",1:500, Akt antibody,"AF6261, Affinity",1:500, Phospho-Akt antibody,"7160, CST",1:1000, Phospho-p70 S6 Kinase antibody,"AF3228, Affinity",1:500, PKCαβ antibody,"ab179522, Abcam",1:1000, Phospho-PKC

(7) Heparan sulfate 6-O-endosulfatases, Sulf1 and Sulf2, regulate glomerular integrity by modulating growth factor signalingAmerican Journal of Physiology-Renal PhysiologyMarch 1, 2016Yasutoshi Takashima, Kazuko Keino-Masu, Hiroshi Yashiro, Satoshi Hara, Tomo Suzuki, Toin H. van Kuppevelt, Masayuki Masu, Michio Nagata[PMSF (1:10)]. The amount of phosphorylated (p-)Akt in isolated glomeruli was determined using an ELISA kit (no. 7160C, Cell Signaling Technology). Statistical analysis. ANOVA with a post hoc Tukey-Kramer honestly significant

(8) Cyclin D1 sensitizes myeloma cells to endoplasmic reticulum stress-mediated apoptosis by activating the unfolded protein response pathwayBMC CancerDecember 1, 2015Sophie Bustany, Julie Cahu, Philippe Guardiola, Brigitte SolaWe used primary Abs against poly(ADP-ribosyl)transferase (or PARP, #9542), caspase 9 (#9508), XBP1 (#7160), eIF2α (#9722), phospho(p)-eIF2α (#9721), and BiP (#3183) from Cell Signaling Tech. (Danvers, MA).

(9) Reciprocal activation between IL-6/STAT3 and NOX4/Akt signalings promotes proliferation and survival of non-small cell lung cancer cellsOncotargetJanuary 8, 2015Juan Li, Tian Lan, Cuixiang Zhang, Cheng Zeng, Jincai Hou, Zhicheng Yang, Min Zhang, Jianxun Liu, Bing Liu391:499–510. ELISA analysis ELISA analysis for Akt, pAkt and IL-6 using the ELISA analysis kits (pAkt: Cat. no. 7160, CST; Akt: Cat. no. 7170, CST; IL-6: Cat. no. D6050, R&D systems) according to the manufacturer’s instructions.

(10) Chemical Induction of Unfolded Protein Response Enhances Cancer Cell Killing through Lytic Virus InfectionJournal of VirologyNovember 15, 2014Vibhu Prasad, Maarit Suomalainen, Mirjam Pennauer, Artur Yakimovich, Vardan Andriasyan, Silvio Hemmi, Urs F. GreberBD Biosciences), anti-IRE-1 (catalog number 3294; Cell Signaling Technology), anti-XBP-1 (catalog number 7160; Santa Cruz), anti-protein VI (17), or anti-␤-tubulin (Amersham) antibodies, washed with Tris-buffered

(11) Clonal culturing of human embryonic stem cells on laminin-521/E-cadherin matrix in defined and xeno-free environmentNature communicationsJanuary 1, 2014Rodin S, Antonsson L, Niaudet C, Simonson OE, Salmela E, Hansson EM, Domogatskaya A, Xiao Z, Damdimopoulou P, Sheikhi M, et al.and total-Erk (#9102) were obtained from Cell Signaling Technology. PathScan Phospho-Akt1 sandwich ELISA kit (#7160), PathScan total Akt1 sandwich ELISA kit (#7170), PathScan Phospho-Akt2 sandwich ELISA kit (#7048) and

(12) Atorvastatin overcomes gefitinib resistance in KRAS mutant human non-small cell lung carcinoma cellsCell death & diseaseSeptember 26, 2013Chen J, Bi H, Hou J, Zhang X, Zhang C, Yue L, Wen X, Liu D, Shi H, Yuan J, et al.filters.35 ELISA analysis ELISA analysis both in vitro and in vivo using the ELISA analysis kits (pAKT: Cat. no. 7160, CST; AKT: Cat. no. 7170, CST; pERK: Cat. no. 7315, CST; ERK: Cat. no. 7050, CST) according to the manufacturer's

(13) Role of the inositol polyphosphate-4-phosphatase type II Inpp4b in the generation of ovarian teratomasDevelopmental BiologyJanuary 1, 2013Ashwini Balakrishnan, J. Richard Chailletlysates using the PathScan® Phospho-Akt1 (Ser473) and Total Akt1 Sandwich ELISA Kit (Cell signaling: 7160 & 7170) according to manufacturer instructions. Statistical analysis Each experiment was repeated

(14) Optimization of a Small Tropomyosin-Related Kinase B (TrkB) Agonist 7,8-Dihydroxyflavone Active in Mouse Models of DepressionJournal of Medicinal ChemistryOctober 11, 2012Xia Liu, Chi-Bun Chan, Qi Qi, Ge Xiao, Hongbo R. Luo, Xiaolin He, Keqiang YeS473 ELISA PathScan Phospho-Akt1 (Ser473) Sandwich ELISA kit was purchased from Cell Signaling (Cat.# 7160). 100 μl sample in sample diluent (supplied in the kit) was added to each well and incubated overnight

(15) Mesenchymal Stem Cells Secrete Multiple Cytokines That Promote Angiogenesis and Have Contrasting Effects on Chemotaxis and ApoptosisPLoS ONEApril 25, 2012Robert A. Boomsma, David L. Geenen(Thr308) and ERK 1/2 using PathScan Sandwich ELISA Kits (Cell Signaling Technology #7162, 7182, 7170, 7160, 7252) or a STAR ERK 1/2 ELISA kit (Upstate #17-463). H9c2 cultures were treated with Mesencult, CM,

(16) Reorienting the Fab domains of trastuzumab results in potent HER2 activatorsPloS oneJanuary 1, 2012Scheer JM, Sandoval W, Elliott JM, Shao L, Luis E, Lewin-Koh SC, Schaefer G, Vandlen R.Sigma). The cell lysates described above were also evaluated with PathScan p-AKT1 (S473) Sandwich ELISA (Cat#7160, Cell Signaling Technology). Protein (10 µg) diluted in sample diluents was added to each well of the

(17) HIV-mediated phosphatidylinositol 3-kinase/serine-threonine kinase activation in APCs leads to programmed death-1 ligand upregulation and suppression of HIV-specific CD8 T cellsJournal of immunology (Baltimore, Md. : 1950)September 15, 2011Muthumani K, Shedlock DJ, Choo DK, Fagone P, Kawalekar OU, Goodman J, Bian CB, Ramanathan AA, Atman P, Tebas P, et al.the manufacturer’s instructions. ELISA for phospho- and total Akt was performed using PathScan® kits (7160 and 7170) from Cell Signaling Technologies (Danvers, MA); the phospho-Akt specific ELISA detects Akt

(18) HCV causes chronic endoplasmic reticulum stress leading to adaptation and interference with the unfolded protein responsePloS oneJanuary 1, 2011Merquiol E, Uzi D, Mueller T, Goldenberg D, Nahmias Y, Xavier RJ, Tirosh B, Shibolet O.antibodies The following antibodies were used: NS5a (Virogen, MA, USA #276-A), XBP-1 (Santa Cruz CA, #7160), total-IRE1 α (Cell Signaling #3294) phospho-IRE1α (Abcam, Cambridge, UK, #48187), phospho/total- eiF2α

(19) A Synthetic 7,8-Dihydroxyflavone Derivative Promotes Neurogenesis and Exhibits Potent Antidepressant EffectJournal of Medicinal ChemistryDecember 9, 2010Xia Liu, Chi-Bun Chan, Sung-Wuk Jang, Sompol Pradoldej, Junjian Huang, Kunyan He, Lien H. Phun, Stefan France, Ge Xiao, Yonghui Jia, et al.62409-024). PathScan Phospho-Akt1 (Ser473) Sandwich ELISA kit was purchased from Cell Signaling (Cat.# 7160). The cell lysates from primary neurons or mouse brain that were treated with various compounds were

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PathScan ® Phospho-Akt1 (Ser473) Sandwich ELISA Kit from Cell Signaling Technology

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PathScan ® Phospho-Akt1 (Ser473) Sandwich ELISA Kit from Cell Signaling Technology

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