Total Exosome Isolation Kit (from plasma) 4484450 from Thermo Fisher Scientific

Product Specs
ItemTotal Exosome Isolation Kit (from plasma)
CompanyThermo Fisher Scientific
Price $315.00 Inquire
Catalog Number4484450
FormatLiquid
TypeTotal Exosome Isolation Kit

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Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

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Website: www.thermofisher.com

(1) Understanding the Mechanisms of Human Liver Regeneration via Characterization of Circulating Extracellular VesiclesBiomed Res IntMarch 8, 2026Yilin Sun, Elisa Pasini, Anh Thu Nguyen-Lefebvre, Mamatha BhatIsolation The Exosome Isolation Kit (from plasma) from Invitrogen (Thermo Fisher Scientific, Cat. No. 4484450) was used to isolate sEVs from patient plasma samples following the manufacturer′s protocol. This kit

(2) P2X7R Signaling and Differential Regulation of Neuroinflammatory and Behavior Responses in Male and Female Mice During Chronic Ethanol ExposureInt J Mol SciMarch 2, 2026Namdev S. Togre, Priyanka S. Bhoj, Naveen Mekala, Jayshil Trivedi, Malika Y. Winfield, Rebecca E. Hancock, Uma Sriram, Slava Rom, Yuri PersidskyNanoparticle Tracking Analysis EVs were isolated from plasma samples using a kit-based protocol (cat. no. 4484450; Invitrogen, Carlsbad, CA, USA) [20,133]. Nanoparticle tracking analysis (NTA) of the isolated EVs was

(3) Circulating Extracellular Vesicles Downregulate NOS3 Expression in Endothelial Cells in Atrial FibrillationJournal of Clinical MedicineFebruary 10, 2026Nyozin Leimon, Anna Suzuki, Kohei Kawajiri, Giichi Nitta, Junji Yamaguchi, Satoshi Iwamiya, Satomi Hamada, Yasuhiro Shirai, Lai Wei, Masahiro Yamazoe, et al.aliquoted to 500 μL each in 1.5 mL tubes. EVs were isolated from 500 μL of plasma samples using the Total Exosome Isolation Kit (from plasma) (Thermo Fisher Scientific, Waltham, MA, USA), according to the manufacturer’s instructions. The EV

(4) HIV-HPV interactions via extracellular vesicles among tobacco smokers and nonsmokersExperimental Biology and MedicineJanuary 21, 2026Namita Sinha, Laree Hiser, Sandip Godse, Lina Zhou, Zhanserik Shynykul, Carolann Risley, Theodore Cory, Santosh Kumarprotocols [26, 34, 35], employing a commercially available precipitation reagent (Invitrogen Catalog number 4484450). The method yields EVs with minimal plasma, including HIV particles, or cellular contaminations, which

(5) Extracellular vesicle-derived miR-760 as a novel promising candidate biomarker differentiating stable RRMS from SPMSScientific ReportsJanuary 14, 2026Karina Wasilewska, Angela Dziedzic, Shamundeeswari Anandan, Elżbieta Miller, Łukasz Łaczmański, Radosław Zajdel, Sylwia Michlewska, Dorota Kujawa, Marta Gancarek, Justyna Raczkowska, et al.unprotected circulating RNA19. EVs were then isolated using the Total Exosome Isolation Kit (plasma) (cat. 4484450; Invitrogen, USA) following the manufacturer’s protocol. The resulting pellet was resuspended in 200

(6) Exclusive Breastfeeding Drives AMPK‐Dependent Thermogenic Memory in BAT and Promotes Long‐Term Metabolic Benefits in OffspringAdvanced ScienceDecember 19, 2025Ningxi Wu, Anwen Yin, Xiang Yu, Mingxin Wang, Jiahui Zhang, Kerong Liu, Yutong Hou, Minkai Cao, Yiting Zhang, Xiaoxiao Zhang, et al.Transfection Breast milk samples were subjected to EV isolation using the Total Exosome Isolation Kit (4484450, Invitrogen, USA), according to the manufacturer's protocol. The isolated EVs were characterized using

(7) Exosomal miR-3126-5p derived from cancer-associated fibroblasts facilitates glycolysis to accelerate NSCLC progression by targeting KLF13 to activate the SH2B1/IRS1 axisClin Transl MedDecember 18, 2025Zhenyu Zhang, Haicheng Ma, Yingying Zheng, Lina Wang, Chenghui Wang, Yuanyuan Liu, Hengxiao Lu, Shaoqiang WangCAFs/NFs culture supernatants and peripheral blood plasma utilizing the total exos isolation kit (4478359/4484450, Thermo Fisher Scientific). For Exos isolation from CAFs/NFs supernatants, the samples were centrifuged

(8) Human plasma-derived eNAMPT-containing extracellular vesicles promote NAD+ biosynthesis and thermogenesis in micenpj AgingNovember 24, 2025Kiyoshi Yoshioka, Takumi Sugimoto, Mamoru Oyabu, Naoki Ito, Aoi Kodama, Yasutomi Kamei, Shin-ichiro Imaiconducted in accordance with the Declaration of Helsinki. For the injection of EVs from mouse plasma, the Total Exosome Isolation Kit from Plasma (ThermoFisher Scientific) was employed based on the manufacturer’s instructions. Mice were injected

(9) eIF4E Enriched Extracellular Vesicles Induce Immunosuppressive Macrophages through HMGCR‐Mediated Metabolic RewiringAdvanced ScienceNovember 1, 2025Sonam Mittal, Minal Nenwani, Ishaque Pulikkal Kadamberi, Sudhir Kumar, Olamide Animasahun, Jasmine George, Shirng‐Wern Tsaih, Prachi Gupta, Mona Singh, Anjali Geethadevi, et al.vesicles. EVs were then purified from the supernatant, using the Exosome Isolation Kit (Invitrogen, Cat# 4484450). The EV size distribution and concentrations were determined by NTA, using the NanoSight LM10 instrument

(10) Moderate Chronic Treadmill Exercise Slows Dopaminergic Neuron Loss in a Rat Model of Parkinson's Disease and Alters RNA Content of Circulating Plasma ExosomesJ Neurosci ResOctober 13, 2025Bruce A. Citron, Maynard Guzman, Bobak Shapdoor, Pranela Rameshwar, Arpine Sokratian, Amaan L. Shaikh, Anya E. Mausoof, Andrew B. West, Vedad DelicHealth catalog# NC1404477), or the Total Exosome Isolation (from plasma) kit (Invitrogen, catalog #4484450) according to manufacturers' instructions. Exosomal precipitation kits from Fisher or Invitrogen resulted

(11) Comparative Proteomics of Seminal Exosomes Reveals Size-Exclusion Chromatography Outperforms UltracentrifugationBiomedicinesOctober 9, 2025Ajaya K. Moharana, Manesh Kumar Panner Selvam, Soumya Ranjan Jena, Partha K. Chandra, David W. Busija, Luna Samanta, Suresh C. SikkaExosomes Using Western Blot Briefly, 40 µL of Invitrogen™ total exosome isolation reagent (Catalog#4484450, Thermo Fisher Scientific, Waltham, MA, USA) was added to 200 µL of SEC-isolated fractions F1–F9 and

(12) Transmission Electron Microscopy-based characterization of Extracellular Vesicles from plasma and serum from Parkinson´s Disease patientsCell Communication and SignalingSeptember 18, 2025Alexander Weiss, Alex Florin Meissner, Fanni Annamária Boros, Martin Regensburger, Regina Verena Taudte, Andreu Matamoros-Angles, Philipp Arnold, Friederike ZunkeFisher´s Total Exosome Isolation Kit (TEIK) (ThermoFisher Scientific Inc., Waltham, MA, United States, #4484450) and the Total Exosome Isolation Reagent (TEIR) (ThermoFisher Scientific Inc., Waltham, MA, United States,

(13) Small Extracellular Vesicles as Biomarkers in Sarcoma Follow-Up: Protocol for a Prospective, Multicentric Pilot StudyJMIR Research ProtocolsSeptember 9, 2025Valentin Vautrot, Alice Hervieu, Aurélie Bertaut, Céline Charon-Barra, Isen Naiken, Sylvain Causseret, Loic Chaigneau, Isabelle Desmoulins, Emilie Rederstoff, Nicolas Isambert, et al.at 2000 g at 4 °C for 20 minutes. Exosomes will be extracted with Total sEV Precipitation Reagent (4484450, Thermo Fisher Scientific) for 10 minutes following homogenization. Part of the EVs will be used to

(14) LncRNA Foxo6os as a Novel “ Scaffold” Mediates MYBPC3 in Combating Pathological Cardiac Hypertrophy and Heart FailureAdvanced ScienceSeptember 1, 2025Jie Sheng, Qin Lin, Yizhuo Sun, Yilei Meng, Sangyu Hu, Huaming Cao, Fang Lin, Yuping Zhu, Luying Peng, Li LiExosomal RNA Exosomes were extracted from plasma using the total exosome isolation kit (from plasma) (#4484450, Thermo Fisher Scientific, Waltham, USA) according to the manufacturer's instructions. Briefly, exosomes

(15) Quantum machine learning-based electrokinetic mining for the identification of nanoparticles and exosomes with minimal training dataBioactive MaterialsSeptember 1, 2025Abhimanyu Thakur, Pedro Correia Santos Bezerra, Abhishek, Shihao Zeng, Kui Zhang, Werner Treptow, Alexander Luna, Urszula Dougherty, Akushika Kwesi, Isabella R. Huang, et al.from plasma were isolated using Total Exosome Isolation kit (from plasma) (Invitrogen, catalog no. 4484450) as per the manufacturer's protocol. The reagent works by sequestering water molecules, which causes

(16) Childhood Asthma Biomarkers Derived from Plasma and Saliva Exosomal miRNAsInternational Journal of Molecular SciencesJuly 22, 2025Abdelnaby Khalyfa, Mohit Verma, Meghan M. Alexander, Zhuanhong Qiao, Tammy Rood, Ragini Kapoor, Trupti Joshi, David Gozal, Benjamin D. Franciscostored at −80 °C. 4.3. Exosome Isolations and Characterization Plasma exosomes were isolated using a Total Exosome Isolation Kit (from plasma, Life Technologies, Marietta, OH, USA) [30,31,61]. Briefly, saliva was thawed at 37 °C and further centrifuged

(17) CD20-bearing extracellular vesicles are associated with prognostic biomarkers of patients with AIDS-NHLScientific reportsJuly 12, 2025Martínez LE, Lensing S, Chang D, Magpantay LI, Guo Y, Mitsuyasu R, Ambinder RF, Sparano JA, Martínez-Maza O, Epeldegui M.0.2 ml volume of the exosome precipitation reagent (from plasma) was added to the sample (Catalog No. 4484450; Invitrogen Total Exosome Isolation Kit (from plasma), Thermo Fisher Scientific, Waltham, MA, USA).

(18) Extracellular Vesicle Metabolomics Holds Promise for Adult Axon RegenerationMetabolitesJuly 4, 2025Maria D. Cabrera Gonzalez, Jackson Watson, Laura Leal, Isabella Moceri, Camille Plummer, Biraj Mahato, Abdelrahman Y. Fouda, Sanjoy K. BhattacharyaIsolation from cell culture,4478359,[39] "ThermoFisher (Waltham, MA, USA)",Total Isolation from plasma,4484450,"[40,41]" "ThermoFisher (Waltham, MA, USA)",Total Isolation from serum,4478360,[39] "ThermoFisher

(19) Pancreatic Cancer‐Derived Extracellular Vesicles Enriched with miR‐223‐5p Promote Skeletal Muscle Wasting Associated with CachexiaAdvanced ScienceJuly 2, 2025Kangjing Xu, Rongxi Shen, Li Zhang, Xuejin Gao, Xinbo Wang, Changhua Zhang, Xi Chen, Xinying Wangprevious research, the EV pellet was collected, and the supernatant was further processed with the Total Exosome Isolation Kit (from plasma) (Invitrogen, 4 484 450) following the manufacturer's instructions.[ 55 ] The protein concentration

(20) Comprehensive Comparison of Methods for Isolation of Extracellular Vesicles from Human PlasmaJournal of Proteome ResearchJune 6, 2025Patil Shivprasad Suresh, Qibin ZhangSmartSEC Single EV isolation kit (cat# SSEC200A-1, System Biosciences), Total exosome isolation kit (cat# 4484450, Invitrogen), Plasma/Serum Exosome Purification Mini-Kit (cat# 57400, Norgen Biotek), MagResyn SAX magnetic

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Total Exosome Isolation Kit (from plasma) from Thermo Fisher Scientific

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Total Exosome Isolation Kit (from plasma) from Thermo Fisher Scientific

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