Fig 1: Immune staining of nuclear p53 before and after isotretinoin treatment. a p53 nuclear immune staining in sebaceous gland of acne patient before isotretinoin therapy. b Intensified nuclear p53 immune staining after 6 weeks of isotretinoin therapy
Fig 2: (A) Schematic diagram of full-length P53 and the NLS-truncated form. (B-C) Analysis of the interaction between the P53 NLS domain and G3BP1. (D) Schematic diagram of the full-length and different truncated forms of G3BP1. (E-F) Analysis of the interaction between the different domains of G3BP1 and P53
Fig 3: Promoting G3BP1 expression could inhibit P53 entry by binding to the nuclear localization sequence of P53. The inhibition of SLC7A11 transcription was weakened after blocking of P53 binding to the promoter region of the SLC7A11 gene. The SLC7A11-GSH-GPX4 antiferroptotic pathway was subsequently activated, and the level of ferroptosis in ALF hepatocytes was inhibited
Fig 4: G3BP1 affects ferroptosis by regulating the SLC7A11-GSH-GPX4 axis through the P53 NLS. (A) G3BP1 mRNA was detected by RT?PCR. (B-G) The levels of cell viability, LDH, ROS, MDA, GSH, and Fe2+ were detected by test kits. (H) P53 protein expression was detected by an ELISA kit. (I) SLC7A11 mRNA was detected by RT?PCR. (J-K) The protein levels of G3BP1, SLC7A11 and GPX4 were determined by western blotting. N = 3. The data are shown \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\bar x$$\end{document} ± s. * P < 0.05, compared with the normal group. # P < 0.05, compared with the model group. ? P < 0.05, compared with the P53-NLS-/- group. & P < 0.05, compared with the siRNA-G3BP1 group
Fig 5: (A-C) The protein levels of G3BP1 and P53 in L02 cells were observed by fluorescence staining. N = 3. The data are shown \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\bar x$$\end{document} ± s. * P < 0.05, compared with the normal group. # P < 0.05, compared with the model group
Supplier Page from Abcam for Human p53 ELISA Kit