Fig 1: Effect of the 2,4-Di-Cl-MGV-1 on NF-κB p65 (pS536) levels (measured by ELISA) in LPS- stimulated BV-2 cells. BV-2 cells were exposed to 100 ng/ml LPS for 24 h with or without 2,4-Di-Cl-MGV-1 or DS (25 μM each). Concentrations (μg/ml) were calculated using a standard calibration curve and are presented as Means ± SD; 4 replicates in each group. ANOVA with Bonferroni’s post-hoc test was performed (p < 0.001 compared to all other groups).
Fig 2: Vemurafenib inhibited mTOR and p70S6K activation in dyW−/− mice. Levels of (A) phosphorylated ERK1/2 (pT202/Y204), (B) phosphorylated STAT3 (pY705), (C) phosphorylated NFκB p65 (pS536), (D) phosphorylated JNK1/2 (pT183/Y185), (E) phosphorylated mTOR (pS2448) and (F) phosphorylated p70S6K (pT389) in protein extracts of gastrocnemius muscle from WT mice, vehicle-treated dyW−/− mice and vemurafenib-treated dyW−/− mice. One-way ANOVA with uncorrected Fisher's LSD test was performed for the data that followed the normal distribution [ERK1/2 (pT202/Y204), mTOR (pS2448) and NFκB p65 (pS536)]. Kruskal–Wallis test was performed for the data that did not follow the normal distribution [STAT3 (pY705), JNK1/2 (pT183/Y185) and p70S6K (pT389)]. All data are represented by statistical significance of mean±s.e.m. (n=10 for all groups). ns, not significant; *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001.
Fig 3: Effects of VTX on Modulation of pro-inflammatory cytokines and NF-κB p65 in the hippocampus and prefrontal cortex after MCAO/R. (A–C) The levels of pro-inflammatory cytokines (TNF-ɑ, IL-1β, IL-6) in the hippocampus and prefrontal cortex after MCAO/R. (D–F) Effect of VTX on NF-κB p65 activation after MCAO/R. NF-κB p65 protein secretion levels in whole cells (D), cytosolic (E), and nuclear (F) were analyzed by ELISA. Data are expressed as the mean ± SEM (n = 3) and were analyzed by one-way ANOVA (post hoc analysis: Tukey’s post hoc analysis test). * (p < 0.05) and ** (p < 0.01) represent significance compared to the I/R group. # (p < 0.05) and ## (p < 0.01) represents significance compared to the CTRL group.
Fig 4: Effects of VTX on the NF-κB p65 protein expression in hippocampus and prefrontal cortex of rats after MCAO/R. (A, B) NF-κB p65 protein secretion levels in whole cells, (C, D) cytosolic, and (E, F) nuclear were analyzed by Western blot. Data are expressed as the mean ± SEM (n = 3) and were analyzed by one-way ANOVA (post hoc analysis: Tukey’s post hoc analysis test). * (p < 0.05) and ** (p < 0.01) represent significance compared to the I/R group. # (p < 0.05) and ## (p < 0.01) represents significance compared to the CTRL group.
Supplier Page from Abcam for NF kappaB p65 (pS536) ELISA Kit