PKC Kinase Activity Assay Kit ab139437 from Abcam

Product Specs
ItemPKC Kinase Activity Assay Kit
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab139437
ApplicationsEnzyme activity assay
Gene NamePRKCG
Assay Time4h 30m
Sample TypeAdherent cells, Suspension cells, Tissue Extracts, Purified protein
Quantity1 x 96 Tests

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Impact of PKC-MAPK Signaling on Cardiac Sympathetic Overactivation in Type-2 Diabetes MellitusInternational Journal of Molecular SciencesJanuary 10, 2026Jaswinder Singh, Afia Saabea Owusu Konadu, Yu Li, Boris Shabaltiy, Yu-Long Lirelative kinase activity in lysates was assessed using the PKC kinase activity assay kit (Cat. No. ab139437, Abcam, Cambridge, UK). TACE activity was quantified in SG extracts using the Sensolyte 520 TACE (alpha

(2) Cardiac sodium-glucose co-transporter 1 (SGLT1) contributes to heart failure in a mouse model of diabetic cardiomyopathyBasic research in cardiologyDecember 1, 2025Li Z, Freiberg S, Music ML, Gu L, Nacos S, Phillips JP, Hassan A, Shibbani K, Munir SS, Kumar VK, et al.h. Protein kinase C (PKC) activity PKC activity was measured with a commercially available kit (Abcam, Ab139437) following the manufacturer’s instructions. Superoxide production To monitor superoxide production

(3) Activity of protein kinase C controls the efficacy of cannabinoid receptor type 1 in the medial prefrontal cortex after neuropathic painJournal of NeurophysiologyDecember 1, 2025Dongman Chao, Yong Liu, Tao Cheng, Quinn H. Hogan, Bin Panbiochemical analyses. Kinase activity was quantified using a colorimetric PKC activity ELISA kit (Abcam, ab139437) following the manufacturer’s protocol. Equal protein (5–10 μg/well) was incubated on PKC substrate-coated

(4) Protein Kinase C promotes peroxisome biogenesis and peroxisome-endoplasmic reticulum interactionJ Cell BiolSeptember 1, 2025Anya Borisyuk, Charlotte Howman, Sundararaghavan Pattabiraman, Daniel Kaganovich, Triana Amenpromoter. PKC activity Protein kinase activity was determined by the PKC Kinase Activity Assay Kit (ab139437; Abcam) according to the manufacturer’s instructions. Protein concentration in lysates was quantified

(5) Covalent Binding of Thioredoxin to TXNIP is Required for Diet-induced Insulin Resistance in the LiverJournal of Biological ChemistryMay 8, 2025Sezin Dagdeviren, Megan F. Hoang, Jialu Wang, Tamara Goldberger, Amelia M. Yu, Steven J. Blair, Jake C. Benoit, Elisabeth M. Ricci-Blair, Veronika Y. Melnik, Bo-Yeon Kim, et al.(Abcam; #ab65341). PKC activity was measured by PKC Kinase Activity Assay Kit in liver lysates (Abcam; #ab139437). The kit measures the activity of all PKC isoforms. Liver triglycerides were measured in 8-week HFD-fed

(6) Melatonin regulates rabbit sperm motility and kinematics via the MT1/PKC signaling pathwayAnimal BioscienceMay 1, 2025Chongchong Wang, Yanyan Zhang, Shiwen He, Biao Jiang, Jinglei Huang, Hui Pengsamples (n = 6) were collected and PKC activity was measured using a PKC Kinase Activity Assay Kit (ab139437; Abcam, Cambridge, UK) following the manufacturer’s protocol. Briefly, the kinase reaction was performed

(7) The Dual Role of A2aR in Neuroinflammation: Modulating Microglial Polarization in White Matter LesionseNeuroFebruary 13, 2025Chang Cheng, Wenchao Cheng, Yuhan Wang, Xiuying Chen, Lan Zhang, Yi Li, Fa Shen, Dezhi Yuan, Pian Hong, Wen Huangassessed using the PKA Kinase Activity Assay Kit (ab139435, Abcam) and PKC Kinase Activity Assay Kit (ab139437, Abcam). Briefly, cells were washed with PBS and lysed with buffer containing 50 mM KH2PO4, 1.5 mM MgCl2,

(8) The Dual Role of A2aR in Neuroinflammation: Modulating Microglial Polarization in White Matter LesionseNeuroFebruary 13, 2025Chang Cheng, Wenchao Cheng, Yuhan Wang, Xiuying Chen, Lan Zhang, Yi Li, Fa Shen, Dezhi Yuan, Pian Hong, Wen Huangassessed using the PKA Kinase Activity Assay Kit (ab139435, Abcam) and PKC Kinase Activity Assay Kit (ab139437, Abcam). Briefly, cells were washed with PBS and lysed with buffer containing 50 mM KH2PO4, 1.5 mM MgCl2,

(9) A protocol for measuring the activity of protein kinase C-delta in murine bone-marrow-derived dendritic cellsSTAR protocolsSeptember 20, 2024Mohati SM, Matak AM, Makdissi S, Di Cara F.gave a good signal. The samples were diluted in Kinase Assay Dilution Buffer (provided in the Abcam AB139437 kit). The step-by-step section below describes how to measure PKC kinase activity using the PKC Kinase Activity Assay Kit

(10) S-nitrosoglutathione reductase alleviates morphine analgesic tolerance by restricting PKCα S-nitrosation☆Redox BiologySeptember 1, 2024Ling-Yan Su, Lijin Jiao, Qianjin Liu, Xinhua Qiao, Ting Xie, Zhiyu Ma, Min Xu, Mao-Sen Ye, Lu-Xiu Yang, Chang Chen, et al.PKCα activity assay The PKCα kinase assay was conducted using a PKC Kinase Activity Assay Kit (Abcam, ab139437) according to the manufacturer's protocols, with some modifications. In brief, cell samples were homogenized

(11) The role of leptin in regulation of the soluble amyloid precursor protein α (sAPPα) levels in lung cancer cell mediaScientific reportsFebruary 28, 2024Haddad B, Khalil J, Al Khashali H, Ray R, Goel S, Darweesh B, Coleman KL, Wozniak C, Ranzenberger R, Lopo B, et al.p53 binding sequence, bound to 96-well plates. PKC assay The PKC kinase activity assay kit (Abcam, ab139437) was used as we previously reported38. In this ELISA, a polyclonal antibody was used to detect phosphorylation

(12) Role of Protein Kinase C in Metabolic Regulation of Coronary Endothelial Small Conductance Calcium‐Activated Potassium ChannelsJournal of the American Heart AssociationFebruary 6, 2024Hang Xing, Sharif A. Sabe, Guangbin Shi, Dwight D. Harris, Yuhong Liu, Frank W. Sellke, Jun Fengthen the supernatant was collected. The levels of PKC in the supernatant were counted by ELISA kit (ab139437, Abcam), following recommended manufacturer's instructions. The concentration was determined by calculating

(13) Role of Protein Kinase C in Metabolic Regulation of Coronary Endothelial Small Conductance Calcium‐Activated Potassium ChannelsJ Am Heart AssocJanuary 31, 2024Hang Xing, Sharif A. Sabe, Guangbin Shi, Dwight D. Harris, Yuhong Liu, Frank W. Sellke, Jun Fengthen the supernatant was collected. The levels of PKC in the supernatant were counted by ELISA kit (ab139437, Abcam), following recommended manufacturer's instructions. The concentration was determined by calculating

(14) Mechanistic Investigation of WWOX Function in NF-kB-Induced Skin Inflammation in PsoriasisInternational journal of molecular sciencesDecember 21, 2023Shin MJ, Kim HS, Lee P, Yang NG, Kim JY, Eun YS, Lee W, Kim D, Lee Y, Jung KE, et al.orthovanadate, and 2.5 mM sodium pyrophosphate). PKC activities in supernatants were assayed using a kit (Abcam, ab139437). Absorbance was measured at 450 nm using a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices,

(15) Empagliflozin prevents oxidative stress in human coronary artery endothelial cells via the NHE/PKC/NOX axisRedox BiolDecember 2, 2023Xiaoling Li, Mengnan Wang, Jan-Ole Kalina, Benedikt Preckel, Markus W. Hollmann, Martin Albrecht, Coert J. Zuurbier, Nina C. Weberfurther use. PKC activity was measured from cell lysates with a PKC Kinase Activity Assay Kit (Abcam, ab139437), following the instruction provides by the manufacturer. 2.3 NOX activity measurement Activity of

(16) Monoterpenoid aryl hydrocarbon receptor allosteric antagonists protect against ultraviolet skin damage in female miceNature communicationsMay 11, 2023Ondrová K, Zůvalová I, Vyhlídalová B, Krasulová K, Miková E, Vrzal R, Nádvorník P, Nepal B, Kortagere S, Kopečná M, et al.kinase C (PKC) inhibition was assayed in HepaG2 cell lysates using a PKC Kinase Activity Assay Kit (ab139437; Abcam). Cells were grown to 90% confluency in a 60 mm dish. After removal of the medium, 1 mL of lysis

(17) Sublethal biochemical, behavioral, and physiological toxicity of extremely low dose of bendiocarb insecticide in Periplaneta americana (Blattodea: Blattidae)Environmental Science and Pollution ResearchFebruary 6, 2023Milena Jankowska, Bartosz Augustyn, Justyna Maliszewska, Barbara Przeździecka, Dawid Kubiak, Olga Chełchowska, Jagoda Kaczorek, Dominik Knop, Kornelia Krajnik, Hanna Kletkiewicz, et al.Supernatant was then used to perform direct immunoassays accordingly to the producer’s instruction: ab139437 for PKC activity and ab139435 for PKA activity (Abcam). Protein kinase level was measured colorimetrically

(18) VEGF promotes diabetic retinopathy by upregulating the PKC/ET/NF-ÎºB/ICAM-1 signaling pathwayEuropean Journal of HistochemistryOctober 27, 2022Meiying Zhang, Min Zhou, Xia Cai, Yan Zhou, Xueling Jiang, Yan Luo, Yue Hu, Rong Qiu, Yanrong Wu, Yuejin Zhang, et al.nuclei-free eyeballs of rats. PKC Kinase activity was analyzed using the PKC Kinase Activity Assay Kit (ab139437; Abcam) according to the manufacturer’s instructions. The protein concentration of the samples and the

(19) Regulation of the Soluble Amyloid Precursor Protein α (sAPPα) Levels by Acetylcholinesterase and Brain-Derived Neurotrophic Factor in Lung Cancer Cell MediaInternational journal of molecular sciencesSeptember 15, 2022Al Khashali H, Ray R, Coleman KL, Atali S, Haddad B, Wareham J, Guthrie J, Heyl D, Evans HG.Assay The PKC activity was quantitated using the kinase activity assay kit (Abcam, Waltham, MA, USA, ab139437) In this solid phase ELISA, phosphorylation of a specific PKC synthetic peptide is detected using a

(20) Mechano-signaling via Piezo1 prevents activation and p53-mediated senescence of muscle stem cellsRedox biologyJune 1, 2022Peng Y, Du J, Günther S, Guo X, Wang S, Schneider A, Zhu L, Braun T.Protein kinase C activity assay PKC activity in MuSCs was measured with a PKC Kinase Activity Assay Kit (ab139437, Abcam). Cells were washed once with PBS and lysed with lysis buffer containing 50 mM KH2PO4, 1.5 mM

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PKC Kinase Activity Assay Kit from Abcam

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PKC Kinase Activity Assay Kit from Abcam

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