Biotin Labeling Kit - NH2 from Kamiya Biomedical Company

Protein Biotin Labeling Kit - NH2

Biotin-NH2 Labeling Kit is primarily used for the preparation of biotin-labeled IgG for enzyme immunoassays (EIA). NH2-reactive Biotin (a component of this kit) has a succinimidyl group (NHS) that reacts with an amino group of IgG and other macromolecules. This kit contains all of the necessary reagents for labeling. The labeling process is simple. Add NH2-reactive Biotin to a IgG solution on a filter membrane and incubate for 10 minutes at 37°C. On the average, 5-8 biotin molecules conjugate to each IgG molecule. Excess biotin molecules can be removed using a filtration kit included in the kit.