Mouse MIF DuoSet ELISA DY1978 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemMouse MIF DuoSet ELISA
CompanyR&D Systems, a Bio-Techne Brand
Price $890.00Supplier Page View Company Product Page
Catalog NumberDY1978
Quantity1 Kit (for 15 Plates)
ApplicationsELISA
Detection Range125.00 - 8000 pg/mL
ReactivityMouse
Detection TargetMIF
StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date
NCBI Full Gene Namemacrophage migration inhibitory factor (glycosylation-inhibiting factor)
NCBI Gene AliasesDER6, GIF, Glif

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) An atlas of ferroptosis-induced secretomesCell death and differentiationNovember 1, 2025Yapici FI, Seidel E, Dahlhaus A, Weber J, Schmidt C, de Britto Chaves Filho A, Yang M, Nenchova M, Güngör E, Stroh J, et al.,R&D Systems,Cat# DY401 Mouse IL-6 DuoSet ELISA,R&D Systems,Cat# DY406 Mouse MIF DuoSet ELISA,R&D Systems,Cat# DY1978 Mouse TNF-alpha DuoSet ELISA,R&D Systems,Cat# DY410 NucleoSpin RNA Kit,Macherey-Nagel,Cat# 740955250 Prostaglandin E2 Parameter Assay Kit

(2) Partial removal of visceral epididymal white adipose tissue in obese Ldlr-/-.Leiden mice impacts adipokine secretion, plasma free fatty acids, and improves cerebrovascular healthPLoS OneOctober 17, 2025Florine Seidel, Martine C. Morrison, Ilse Arnoldussen, Vivienne Verweij, Simon Ebert, Joline Attema, Christa de Ruiter, Wim van Duyvenvoorde, Jessica Snabel, Bram Geenen, et al.(MIF) and plasminogen activator inhibitor 1 (PAI-1) at t = 27 weeks (#DY498−05, #DY1119, #DY1069, #DY1978, #DY3828−05 kits respectively, all R&D Systems, Minneapolis, MN, USA). At t = 27 weeks, plasma S100-B

(3) Hypoglycaemic stimulation of macrophage cytokine release is suppressed by AMP‐activated protein kinase activationDiabetic MedicineDecember 24, 2024Jiping Zhang, Alice E. Pollard, Eleanor F. Pearson, David Carling, Benoit Viollet, Kate L. J. Ellacott, Craig Beallsecreted into culture supernatants were measured by ELISA according to the manufacturers' protocols (DY1978, Biotechne, Abingdon, UK). Briefly, media supernatants were collected and diluted, as required. Extracellular

(4) Downregulation of adipose LPL by PAR2 contributes to the development of hypertriglyceridemiaJCI insightJuly 8, 2024Huang Y, Chen L, Li L, Qi Y, Tong H, Wu H, Xu J, Leng L, Cheema S, Sun G, et al.

(5) Olaparib Attenuates Demyelination and Neuroinflammation in an Organotypic Slice Culture Model of Metachromatic LeukodystrophyNeurotherapeutics : the journal of the American Society for Experimental NeuroTherapeuticsSeptember 1, 2023Mekhaeil M, Conroy MJ, Dev KK.mouse IL-17A kit (ELISA Genie; MOFI01286), mouse CCL3 (R&D Systems; DY450), mouse MIF (R&D Systems; DY1978), and mouse IFN-γ (ELISA Genie; MOFI00047). Quantification of secreted IL-6, TNF-α, CCL3, and MIF in

(6) 5-HMF attenuates inflammation and demyelination in experimental autoimmune encephalomyelitis mice by inhibiting the MIF-CD74 interactionActa biochimica et biophysica SinicaJuly 10, 2023Guan D, Li Y, Cui Y, Zhao H, Dong N, Wang K, Ren D, Song T, Wang X, Jin S, et al.Enzyme-linked immunosorbent assay (ELISA) The ELISA kits for TNF-α (MTA00B; R&D Systems), IL-10 (DY417; R&D Systems) and MIF (DY1978; R&D Systems) were used to measure the levels of TNF-α and IL-10 in BV2 cell supernatants and mouse

(7) A pref-1-controlled non-inflammatory mechanism of insulin resistanceiScienceJune 16, 2023Huang Y, Cui D, Chen L, Tong H, Wu H, Muller GK, Qi Y, Wang S, Xu J, Gao X, et al.Mouse Pref-1/DLK-1/FA1 ELISA,RayBiotech,ELM-PREF1-1 Mouse Pref-1/DLK-1/FA1 ELISA Kit,Invitrogen,EM66RB Mouse MIF DuoSet ELISA,R&D SYSTEMS,DY1978 Human MIF DuoSet ELISA,R&D SYSTEMS,DY289 DuoSet ELISA Ancillary Reagent Kit 2,R&D SYSTEMS,DY008 TG

(8) Translational characterization of the temporal dynamics of metabolic dysfunctions in liver, adipose tissue and the gut during diet-induced NASH development in Ldlr−/−.Leiden miceHeliyonMarch 1, 2023Eveline Gart, Wim van Duyvenvoorde, Jessica M. Snabel, Christa de Ruiter, Joline Attema, Martien P.M. Caspers, Serene Lek, Bertie Joan van Heuven, Arjen G.C.L. Speksnijder, Martin Giera, et al.same liver homogenates cytokine MIF was measured following manufacturers protocol (Mouse MIF duoset DY1978, R&D systems, Abingdon, UK). Total protein concentrations were measured with the BCA Protein Assay Kit

(9) Inducing trained immunity in pro-metastatic macrophages to control tumor metastasisNature immunologyFebruary 1, 2023Ding C, Shrestha R, Zhu X, Geller AE, Wu S, Woeste MR, Li W, Wang H, Yuan F, Xu R, et al.supernatants were stored at −80 °C in aliquots. MIF levels were measured by MIF ELISA kit (R&D Systems, DY1978). For anti-MIF neutralizing assay, WGP-trained and untrained peritoneal macrophages were restimulated

(10) Therapeutic Intervention with Anti-Complement Component 5 Antibody Does Not Reduce NASH but Does Attenuate Atherosclerosis and MIF Concentrations in Ldlr-/-.Leiden MiceInternational journal of molecular sciencesSeptember 14, 2022Seidel F, Kleemann R, van Duyvenvoorde W, van Trigt N, Keijzer N, van der Kooij S, van Kooten C, Verschuren L, Menke A, Kiliaan AJ, et al.CUSABIO, Houston, TX, USA). In the same plasma samples, MIF was quantified with the MIF DuoSet ELISA kit (DY1978, R&D Systems, Minneapolis, MN, USA). The concentrations of MCP-1 and adhesion molecules were analyzed

(11) Heat-Inactivated Akkermansia muciniphila Improves Gut Permeability but Does Not Prevent Development of Non-Alcoholic Steatohepatitis in Diet-Induced Obese Ldlr-/-.Leiden MiceInternational journal of molecular sciencesFebruary 19, 2022Morrison MC, Gart E, Duyvenvoorde WV, Snabel J, Nielsen MJ, Leeming DJ, Menke A, Kleemann R.

(12) A high docosahexaenoic acid diet alters lung inflammation and recovery following repetitive exposure to aqueous organic dust extractsThe Journal of nutritional biochemistryNovember 1, 2021Ulu A, Burr A, Heires AJ, Pavlik J, Larsen T, Perez PA, Bravo C, DiPatrizio NV, Baack M, Romberger DJ, et al.

(13) Autophagy inhibition in breast cancer cells induces ROS-mediated MIF expression and M1 macrophage polarizationCellular signallingOctober 1, 2021Cotzomi-Ortega I, Nieto-Yañez O, Juárez-Avelar I, Rojas-Sanchez G, Montes-Alvarado JB, Reyes-Leyva J, Aguilar-Alonso P, Rodriguez-Sosa M, Maycotte P.

(14) Macrophage miR-210 induction and metabolic reprogramming in response to pathogen interaction boost life-threatening inflammationScience advancesMay 1, 2021Virga F, Cappellesso F, Stijlemans B, Henze AT, Trotta R, Van Audenaerde J, Mirchandani AS, Sanchez-Garcia MA, Vandewalle J, Orso F, et al.levels were analyzed using a commercial mouse TNF- DuoSet (DY410, R&D Systems), mouse MIF DuoSet (DY1978, R&D Systems), and murine IL-10 DuoSet (DY217, R&D Systems) ELISA kit, respectively, according to the

(15) Proteomics reveals distinct mechanisms regulating the release of cytokines and alarmins during pyroptosisCell reportsMarch 9, 2021Phulphagar K, Kühn LI, Ebner S, Frauenstein A, Swietlik JJ, Rieckmann J, Meissner F.

(16) MIF inhibition as a strategy for overcoming resistance to immune checkpoint blockade therapy in melanomaOncoImmunologyDecember 21, 2020Ricardo A. de Azevedo, Einav Shoshan, Shanzhi Whang, Gal Markel, Ashvin R. Jaiswal, Arthur Liu, Michael A. Curran, Luiz R. Travassos, Menashe Bar-Elimicroliters was used for MIF quantification using the Mouse MIF DuoSet Sandwich ELISA kit (R&D Systems DY1978) in accordance with the manufacturer’s instructions. Samples were read in an Epoch microplate spectrophotometer

(17) Autophagy Inhibition Induces the Secretion of Macrophage Migration Inhibitory Factor (MIF) with Autocrine and Paracrine Effects on the Promotion of Malignancy in Breast CancerBiologyJanuary 18, 2020Cotzomi-Ortega I, Rosas-Cruz A, Ramírez-Ramírez D, Reyes-Leyva J, Rodriguez-Sosa M, Aguilar-Alonso P, Maycotte P.Migration Inhibitory Factor (MIF) Secretion Secreted MIF was evaluated with a Duo Set mouse MIF ELISA kit (DY1978, R&D systems, MN, USA), according to the manufacturer’s protocol. Cells were plated at a density of

(18) Simvastatin Posttreatment Controls Inflammation and Improves Bacterial Clearance in Experimental SepsisMediators of inflammationJanuary 1, 2020de Jesus Oliveira FM, Gonçalves-de-Albuquerque CF, de Moraes IMM, Reis PA, Rocha VN, Bozza PT, Silva AR, de Castro Faria Neto HC.

(19) Identification of an Increased Alveolar Macrophage Subpopulation in Old Mice That Displays Unique Inflammatory Characteristics and Is Permissive toMycobacterium tuberculosisInfectionThe Journal of ImmunologyOctober 15, 2019William P. Lafuse, Murugesan V. S. Rajaram, Qian Wu, Juan I. Moliva, Jordi B. Torrelles, Joanne Turner, Larry S. SchlesingerThermo Fisher Scientific). ELISA kits were purchased from R&D Systems (CCL2 [DY429], IL-1b [DY421], MIF [DY1978], TNF-a [DY410], and IL-6 [Dy406]), MABTECH (IL-10 [34320]), and PBL Assay Sciences (IFN-b [4210 Verikine-HS

(20) Macrophage migration inhibitory factor enhances influenza-associated mortality in miceJCI insightJuly 11, 2019Smith CA, Tyrell DJ, Kulkarni UA, Wood S, Leng L, Zemans RL, Bucala R, Goldstein DR.(PBL assay science, catalog 42410-1), IL-28 (Invitrogen, catalog 50-246-674), and MIF (R&D Systems DY1978). Assays were then conducted according to each manufacturer’s specifications. Immunoblotting. For immunoblotting

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MIF ELISA

Mouse MIF DuoSet ELISA

Mouse MIF DuoSet ELISA from R&D Systems, a Bio-Techne Brand

R&D Systems, a Bio-Techne Brand

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Mouse MIF DuoSet ELISA from R&D Systems, a Bio-Techne Brand

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