Human Amphiregulin Quantikine ELISA Kit DAR00 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemHuman Amphiregulin Quantikine ELISA Kit
CompanyR&D Systems, a Bio-Techne Brand
Price $680.00Supplier Page View Company Product Page
Catalog NumberDAR00
Quantity1 Kit
ApplicationsELISA
Detection Range15.60 - 1000 pg/mL
ReactivityHuman
Sample TypeCell Culture Supernates (50 uL), Cell Lysates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Saliva (50 uL), Urine (50 uL)
Sensitivity3.56 pg/mL
Detection TargetAmphiregulin
Assay Time4.5 hours
StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date
NCBI Full Gene Nameamphiregulin
NCBI Gene AliasesAR, AREGB, CRDGF, SDGF

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Senescence-Driven Inflammation and Immune Dynamics in the Progression of Radiation CystitisCellsFebruary 13, 2026Sabrina Mota, Austin Goodyke, Elijah P. Ward, Rani Mahyoob, Yung-Chun Lee, Sarah N. Bartolone, Alyssa Mularski, Michael B. Chancellor, Bernadette M. M. Zwaanswas measured separately using enzyme-linked immunosorbent assay (R&D Systems, Minneapolis, MN, USA #DAR00). Irradiated and age-matched control bladders (N = 4/group) were harvested at 1 week, 3 months, and

(2) Targeted proteomics reveals immune protein patterns in serum from patients with calcific aortic stenosisiScienceDecember 19, 2025Alice Mallia, Andrea Binatti, Diego Sangiorgi, Elisa Mikus, Giulia G. Papaianni, Stefania Ghilardi, Erica Gianazza, Francesco Vieceli Vieceli Dalla Sega, Elena Tremoli, Cristina BanfiAmphiregulin was measured with the Quantikine Human Amphiregulin Immunoassay (R&D Systems, Catalog #DAR00), a 4.5-h quantitative sandwich ELISA with a sensitivity of 1.43 pg/mL. Plexin-A4 was assessed using

(3) Quantitative adverse outcome pathway modeling for cigarette-smoke-induced airway mucus hypersecretion. Part 1: adverse-outcome-pathway-based in vitro assessment with repeated exposure to whole cigarette smokeFront ToxicolMay 15, 2025Sakuya Ichikawa, Shugo Muratani, Keigo Sano, Kazuo Erami, Akina Mori, Risa Matsumoto, Shigeaki Itoenzyme-linked immunosorbent assay (ELISA) kit (R&D Systems, Minneapolis, MN, United States, cat. no. DAR00) as per manufacturer instructions. The absorbance was acquired at 450 nm using the Cytation5 system.

(4) 17β-Estradiol Promotes Tumorigenicity Through an Autocrine AREG/EGFR Loop in ER-α-Positive Breast Cancer CellsCellsMay 12, 2025Yoon SY, Jeong Y, Ryu JM, Lee SK, Chae BJ, Yu J, Kim SW, Nam SJ, Kim S, Lee JE.the serum (50 μL) of ER+ breast cancer patients were analyzed using the Human Amphiregulin ELISA Kit (DAR00, R&D Systems) and the Estradiol Parameter Assay Kit (KGE014, R&D Systems), according to the manufacturer’s

(5) Basal cell adhesion molecule (BCAM) promotes mesothelial-to-mesenchymal transition and tumor angiogenesis through paracrine signalingCell communication and signaling : CCSMarch 13, 2025Sivakumar S, Lieber S, Dietze R, Beutgen VM, Sutor EC, Heidemann S, Finkernagel F, Teply-Szymanski J, Nist A, Stiewe T, et al.were quantified by enzyme-linked immunosorbent assay (ELISA), using the following kits: AREG (Cat. #DAR00, R&D Systems/Bio-Techne), FGF2 (Cat. #DFB50, R&D Systems/Bio-Techne), VEGF-A (Cat. #BMS277-2, Life/Thermo Fisher

(6) AREG Upregulation in Cancer Cells via Direct Interaction with Cancer-Associated Fibroblasts Promotes Esophageal Squamous Cell Carcinoma Progression Through EGFR-Erk/p38 MAPK SignalingCellsOctober 19, 2024Takashi Nakanishi, Yu-ichiro Koma, Shoji Miyako, Rikuya Torigoe, Hiroki Yokoo, Masaki Omori, Keitaro Yamanaka, Nobuaki Ishihara, Shuichi Tsukamoto, Takayuki Kodama, et al.collected and analyzed for AREG protein concentration using a human amphiregulin Quantikine ELISA kit (#DAR00, R&D Systems) following the manufacturer’s protocol. The optical density of each well was measured at

(7) Identifying immune signatures of sepsis to increase diagnostic accuracy in very preterm babiesNature communicationsJanuary 9, 2024Das A, Ariyakumar G, Gupta N, Kamdar S, Barugahare A, Deveson-Lucas D, Gee S, Costeloe K, Davey MS, Fleming P, et al.samples from 23 preterm babies with the Human Amphiregulin Quantikine ELISA Kit (R&D systems; cat no: DAR00) following manufacturer’s instructions. Additionally, the following cytokines and chemokines were assessed

(8) Oncogenic KRAS mutation confers chemoresistance by upregulating SIRT1 in non-small cell lung cancerExperimental & Molecular MedicineOctober 2, 2023Dong Hoon Shin, Jeong Yeon Jo, Minyoung Choi, Kyung-Hee Kim, Young-Ki Bae, Sang Soo Kim(Houston, TX, USA). Additionally, a human HB-EGF ELISA kit (DHBEG0) and a human Amphiregulin ELISA kit (DAR00) were purchased from R&D Systems (Abingdon, OX, UK). Transfection and establishment of stable cell

(9) EGF, TGF-α and Amphiregulin Differently Regulate Endometrium-Derived Mesenchymal Stromal/Stem CellsInternational journal of molecular sciencesAugust 29, 2023Kamentseva RS, Kharchenko MV, Gabdrahmanova GV, Kotov MA, Kosheverova VV, Kornilova ES.secreted AREG and IGFBP-1 were quantified in the cell supernatants by Amphiregulin Human ELISA Kit (#DAR00, R&D Systems, USA) and Human IGFBP-1 ELISA Kit (#DGB100, R&D Systems, USA). The data were normalized

(10) Searching for Biomarkers in Proliferative Diabetic Retinopathy: Amphiregulin and ProgranulinMedeniyet Medical JournalDecember 28, 2022Burak Bilgin, Mete Guler, Hulya Cicek, Selma Urfalioglu, Gokhan Kokusari, Bedia Marangozoglu Sahinkit, according to the manufacturer’s guidelines (Quantikine R&D Systems, MN, USA) with catalog nos. DAR00 and DPGRN0, respectively. The program of the Biotek_ELx808 (Winooski, Vermont, USA) device was used

(11) Luteinizing hormone stimulates the expression of amphiregulin in human theca cellsJournal of ovarian researchDecember 7, 2022Liu Y, Zhong Y, Shen X, Guo X, Wu R, Yang T, Chen M.frozen at -20 °C for subsequent measurement of AREG by the Human Amphiregulin Quantikine ELISA Kit (DAR00, R&D Systems, Inc. Minneapolis, MN, USA) according to the manufacturer’s instructions. The detection limit

(12) iRhom2 regulates ERBB signalling to promote KRAS-driven tumour growth of lung cancer cellsJournal of cell scienceSeptember 1, 2022Sieber B, Lu F, Stribbling SM, Grieve AG, Ryan AJ, Freeman M.amphiregulin in the supernatant was determined using the Human Amphiregulin Quantikine ELISA Kit (R&D Systems, DAR00) according to the manufacturer's instructions. In parallel, the cells were lysed in Triton X-100 lysis

(13) Unraveling the differential impact of PAHs and dioxin-like compounds on AKR1C3 reveals the EGFR extracellular domain as a critical determinant of the AHR responseEnvironment internationalJanuary 1, 2022Vogeley C, Sondermann NC, Woeste S, Momin AA, Gilardino V, Hartung F, Heinen M, Maaß SK, Mescher M, Pollet M, et al.

(14) Amphiregulin can predict treatment resistance to palliative first-line cetuximab plus FOLFIRI chemotherapy in patients with RAS wild-type metastatic colorectal cancerScientific reportsDecember 10, 2021Kim SA, Park H, Kim KJ, Kim JW, Sung JH, Nam M, Lee JH, Jung EH, Suh KJ, Lee JY, et al.samples were quantified using a Quantikine Human AREG ELISA (enzyme-linked immunosorbent assay) kit (DAR00, R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s protocol. Cell culture The human

(15) Secreted matrix metalloproteinase-14 is a predictor for antifibrotic effect of IC-2-engineered mesenchymal stem cell sheets on liver fibrosis in miceRegenerative therapyDecember 1, 2021Fukushima K, Itaba N, Kono Y, Okazaki S, Enokida S, Kuranobu N, Murakami J, Enokida M, Nagashima H, Kanzaki S, et al.

(16) AXL cooperates with EGFR to mediate neutrophil elastase-induced migration of prostate cancer cellsiScienceNovember 19, 2021Xiao Z, Hammes SR.cellular debris, and frozen at -80°C in aliquots. Amphiregulin protein was measured using sandwich ELISA (DAR00, R&D Systems). The absorbance at 450 nm was read with a reference wavelength of 540 nm using an ELISA

(17) Pulmonary fibrosis distal airway epithelia are dynamically and structurally dysfunctionalNature communicationsJuly 27, 2021Stancil IT, Michalski JE, Davis-Hall D, Chu HW, Park JA, Magin CM, Yang IV, Smith BJ, Dobrinskikh E, Schwartz DA.

(18) The Combination of Gefitinib With ATRA and ATO Induces Myeloid Differentiation in Acute Promyelocytic Leukemia Resistant CellsFrontiers in oncologyJanuary 1, 2021de Almeida LY, Pereira-Martins DA, Weinhäuser I, Ortiz C, Cândido LA, Lange AP, De Abreu NF, Mendonza SES, de Deus Wagatsuma VM, Do Nascimento MC, et al.measured with the Human EGF Quantikine ELISA Kit (#DEGFR0) and Human Amphiregulin Quantikine ELISA Kit (#DAR00; both from R&D Systems, Minneapolis, MN, USA), respectively, according to the manufacturer’s instructions. PCR

(19) Glutamine deprivation alters the origin and function of cancer cell exosomesThe EMBO journalAugust 17, 2020Fan SJ, Kroeger B, Marie PP, Bridges EM, Mason JD, McCormick K, Zois CE, Sheldon H, Khalid Alham N, Johnson E, et al.in the EV samples was measured using Quantikine ELISA—Human amphiregulin Immunoassay (R&D SYSTEMS; DAR00) according to the manufacturer's instructions. Immmunostaining of cultured human cells For immunofluorescence

(20) Long-lasting severe immune dysfunction in Ebola virus disease survivorsNature communicationsJuly 24, 2020Wiedemann A, Foucat E, Hocini H, Lefebvre C, Hejblum BP, Durand M, Krüger M, Keita AK, Ayouba A, Mély S, et al.

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Amphiregulin ELISA

Human Amphiregulin Quantikine ELISA Kit

Amphiregulin ELISA

Human Amphiregulin Quantikine ELISA Kit from R&D Systems, a Bio-Techne Brand

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R&D Systems, a Bio-Techne Brand

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Human Amphiregulin Quantikine ELISA Kit from R&D Systems, a Bio-Techne Brand

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