Human PD-L1 DuoSet ELISA from R&D Systems, a Bio-Techne Brand

Product Specs
ItemHuman PD-L1 DuoSet ELISA
CompanyR&D Systems, a Bio-Techne Brand
Price $890.00Supplier Page View Company Product Page
Catalog NumberDY156
ApplicationsELISA
NCBI Full Gene NameCD274 molecule
NCBI Gene AliasesB7-H, B7H1, PD-L1, PDCD1L1, PDCD1LG1, PDL1, hPD-L1
StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date
TargetPD-L1/B7-H1
ReactivityHuman
Range156.25 - 10000 pg/mL
Quantity1 Kit (for 15 Plates)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) CD5L insufficiency exacerbates skeletal joint damage in rheumatoid arthritisMolecular MedicineMarch 24, 2026Diana Bicho, Malin C. Erlandsson, David Svensson, Emma Comas, Maria Carolina Silva, Venkataragavan Chandrasekaran, Victoria M. E. Lundgren, Rita F. Santos, Liliana Oliveira, Maria I. Bokarewa, et al.R&D Systems; detection limit, 3 pg/ml), IFN-γ (DY285B, R&D Systems; detection limit, 5 pg/ml), and PD-L1 (DY156, R&D Systems; detection limit, 0.5 pg/ml). In cohort 1, IGF-1 levels were measured by an ELISA using

(2) Engineered Fenretinide- and Tocilizumab-Releasing Janus Nanoparticles for Site-Directed Immunochemoprevention of Squamous Cell Carcinoma of the LungPharmaceuticsNovember 14, 2025Daren Wang, Albert Chang, Fortune Shea, Yifei He, Richard Spinney, Jonathan D. Whitsett, Joerg Lahann, Susan R. Malleryof nuclear PD-L1 in the H520 cells were insufficient for detection by immunoblotting, a PD-L1 ELISA (DY156, R&D Systems, Minneapolis, MN, USA) was conducted with results reported as pg/106 cells. 2.10. In Vivo

(3) Wuhan strain of SARS-CoV-2 triggers activation of immune evasion machinery similar to the one operated by cancer cellsFrontiers in ImmunologyJune 18, 2025Maryam Abooali, Inna M. Yasinska, Gauri Thapa, Xi Lei, Kelly A. S. da Costa, Stephanie Schlichtner, Steffen M. Berger, Elizaveta Fasler-Kan, Nigel J. Temperton, Romina Vuono, et al.lysates by ELISA using R&D Systems kits (DY2045 for galectin-9, DY240 for TGF-β, DY202 for IL-2, and DY156 for PD-L1) according to the manufacturer’s protocols. On-cell Western analysis Cell surface levels

(4) Exosomal PDL1 Suppresses the Anticancer Activity of CD8(+) T Cells in Hepatocellular CarcinomaAnalytical cellular pathology (Amsterdam)January 1, 2024Hu Q, Chen S, Deng R, Deng H, Peng M, Wang X, Deng S, Wang J, Xu B, Xu Y, et al.volunteers. 2.2. ELISA Assay ELISA plates (96-well) (Biolegend) that coated with Human PDL1 antibody (DY156, R&D SYSTEMS, Minneapolis, MN, USA), Human TNFα ELISA Kit (KIT10602, SinoBiology, Beijing, China), Mouse

(5) Ex vivo pleural effusion cultures to study chimeric antigen receptor T cell cytotoxicity in an immunocompetent environmentCell Rep MethodsNovember 20, 2023Tano ZE, Kiesgen S, Chintala NK, Dozier J, Quach HT, Messinger J, Tan KS, Adusumilli PS.as per manufacturer’s instructions. Human PD1 DuoSet (DY1086, R&D systems), and human PDL1 DuoSet (DY156, R&D systems) ELISA kits were run on the MSD Mesoscale Quickplex SQ120 platform (MSD Mesoscale Diagnostics,

(6) Analysis of the Prognostic Potential of Schlafen 11, Programmed Death Ligand 1, and Redox Status in Colorectal Cancer PatientsInternational journal of molecular sciencesOctober 11, 2023Miladinov M, Rosic J, Eric K, Guzonjic A, Jelenkovic J, Bogavac-Stanojevic N, Dimitrijevic I, Kotur-Stevuljevic J, Barisic G.

(7) Identification of a novel cord blood NK cell subpopulation expressing functional programmed death receptor-1Frontiers in ImmunologyJune 22, 2023Marco Greppi, Valentina Obino, Rayan Goda, Federico Rebaudi, Simona Carlomagno, Mariella Della Chiesa, Simona Sivori, Gianluca Ubezio, Vanessa Agostini, Alessandra Bo, et al.CMV in human serum. Soluble PD-L1 was evaluated with Human PD-L1 DuoSet ELISA (R&D System; Catalog #: DY156) following the kit procedure. 2.7 Degranulation assay and IFN-γ production These experiments were

(8) Clinical significance of serum-derived exosomal PD-L1 expression in patients with advanced pancreatic cancerBMC cancerMay 1, 2023Park SJ, Park JY, Shin K, Hong TH, Lee M, Kim Y, Kim IH.(ELISA) The expression of PD-L1 in serum-derived exosomes was detected using human PD-L1 DuoSet ELISA kits (#DY156, R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s protocols. The optical density was

(9) Robust Preanalytical Performance of Soluble PD-1, PD-L1 and PD-L2 Assessed by Sensitive ELISAs in BloodBiomedicinesOctober 11, 2022Krueger K, Mayer Z, Kottmaier M, Gerckens M, Boeck S, Luppa P, Holdenrieder S.standards were purchased from R&D Systems (Human PD-1 DuoSet® ELISA: DY1086/Human PD-L1 DuoSet® ELISA: DY156/Human PD-L2/B7-DC DuoSet® ELISA: DY1224, R&D Systems, Inc., Minneapolis, MN, USA) to create the assay.

(10) High Quality Performance of Novel Immunoassays for the Sensitive Quantification of Soluble PD-1, PD-L1 and PD-L2 in BloodBiomedicinesSeptember 26, 2022Krueger K, Mayer Z, Gerckens M, Boeck S, Luppa P, Holdenrieder S.purchased from DuoSet® Development Systems (Human PD-1 DuoSet® ELISA: DY1086/ Human PD-L1 DuoSet® ELISA: DY156/ Human PD-L2/B7-DC DuoSet® ELISA: DY1224, R&D Systems, Inc., Minneapolis, MN, USA). The standard curves

(11) Oxidative stress and inflammation parameters-novel biomarkers for idiopathic pulmonary fibrosisEuropean review for medical and pharmacological sciencesFebruary 1, 2022Roksandic Milenkovic M, Klisic A, Ceriman V, Kotur Stevuljevic J, Savic Vujovic K, Mirkov D, Gajic M, Ilic B, Dimic N, Samardzic N, et al.

(12) Canine Transforming Growth Factor-Î² Receptor 2-Ig: A Potential Candidate Biologic for Melanoma Treatment That Reverses Transforming Growth Factor-Î²1 ImmunosuppressionFrontiers in Veterinary ScienceJune 14, 2021Hiroto Takeuchi, Satoru Konnai, Naoya Maekawa, Satoshi Takagi, Hiroshi Ohta, Noboru Sasaki, Sangho Kim, Tomohiro Okagawa, Yasuhiko Suzuki, Shiro Murata, et al.TGF-β1 concentration in cell culture supernatants and sera were measured by human TGFβ1 DuoSet ELISA (DY156, R&D systems, Minneapolis, MN). The absorbance was measured at 450 nm with an MTP-900Lab (CORONA ELECTRIC,

(13) Serum and Tissue Expression Levels of Leptin and Leptin Receptor Are Putative Markers of Specific Feline Mammary Carcinoma SubtypesFrontiers in Veterinary ScienceFebruary 10, 2021Andreia Gameiro, Catarina Nascimento, Ana Catarina Urbano, Jorge Correia, Fernando Ferreiracommercial ELISAbased kits (R&D Systems, Minneapolis, USA; DY398-05, DY389, DY476, DY2586, DY1086, and DY156, respectively). For each Number of animals (%) Size Age <8 years old 4 (6.9%) ≥8 years old 54

(14) Soluble PD-L1 Is an Independent Prognostic Factor in Clear Cell Renal Cell CarcinomaCancersFebruary 7, 2021Larrinaga G, Solano-Iturri JD, Errarte P, Unda M, Loizaga-Iriarte A, Pérez-Fernández A, Echevarría E, Asumendi A, Manini C, Angulo JC, et al.soluble PD-L1 and PD-1 were evaluated using the human B7-H1 and PD-1 DuoSet ELISA kits (R&D Systems, DY156 and DY1086, respectively) according to the manufacturer’s protocols [37]. Briefly, 96 well plates were

(15) Distinct roles of programmed death ligand 1 alternative splicing isoforms in colorectal cancerCancer scienceJanuary 1, 2021Wang C, Weng M, Xia S, Zhang M, Chen C, Tang J, Huang D, Yu H, Sun W, Zhang H, et al.ELISA The concentrations of PD‐L1 in the serum were detected using an ELISA kit (R&D Systems, Cat# DY156) according to the manufacturer’s instructions. A total of 2 × 105 PD‐L1 isoform‐overexpressing tumor

(16) Are the Immune Properties of Mesenchymal Stem Cells from Wharton's Jelly Maintained during Chondrogenic Differentiation?Journal of clinical medicineFebruary 4, 2020Voisin C, Cauchois G, Reppel L, Laroye C, Louarn L, Schenowitz C, Sonon P, Poras I, Wang V, D Carosella E, et al.

(17) Membrane PD-L1 expression and soluble PD-L1 plasma levels in idiopathic pulmonary fibrosis-a pilot studyJournal of thoracic diseaseDecember 1, 2018Jovanovic D, Roksandic Milenkovic M, Kotur Stevuljevic J, Markovic J, Ceriman V, Kontic M, Skodric Trifunovic V.

(18) Smoking Is Associated With Low Levels of Soluble PD-L1 in Rheumatoid ArthritisFrontiers in ImmunologyJuly 27, 2018Caroline Wasén, Malin C. Erlandsson, Apostolos Bossios, Linda Ekerljung, Carina Malmhäll, Sofia Töyrä Silfverswärd, Rille Pullerits, Bo Lundbäck, Maria I. Bokarewain PBS supplemented with 1% bovine serum albumin (1% BSA–PBS) using human PD-L1/B7-H1 DuoSet ELISA (DY156, R&D Systems). The protocol was optimized with respect to antibody concentration. The mouse anti-human

(19) Regulation of programmed-death ligand in the human head and neck squamous cell carcinoma microenvironment is mediated through matrix metalloproteinase-mediated proteolytic cleavageInternational Journal of OncologyDecember 11, 2017Mayuko Hira-Miyazawa, Hiroyuki Nakamura, Mariko Hirai, Yutaka Kobayashi, Hiroko Kitahara, George Bou-Gharios, Shuichi Kawashirimembrane-associated proteins were analysed for the presence of PD-L1 using the ELISA kit (cat. no. DY156; R&D Systems Europe Ltd., Abingdon, UK), in accordance with the manufacturer's instructions. The total

(20) Mesenchymal Stromal Cell Secretion of Programmed Death-1 Ligands Regulates T Cell Mediated ImmunosuppressionStem CellsMarch 1, 2017Davies LC, Heldring N, Kadri N, Le Blanc K.

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