Fig 2: Myeloid cell-specific lipin-1 deficiency reduced circulating levels of lipocalin-2 (Lcn2) and serum amyloid A1 (Saa1) in mice after ethanol feeding.Wild-type (WT) or mLipin-1KO (KO) mice were pair-fed either a control diet or an ethanol-containing diet for 10 days followed by single gavage of ethanol. (A) Relative liver mRNA levels of Lcn2 and Saa1. (B) Representative Western analysis of liver Lcn2 and Saa1. (C) Relative liver protein levels of Lcn2 and Saa1. (D) Relative serum Lcn2 levels. (E) Relative serum Saa1 levels. Results are expressed as means ± SEM (n = 4–6 mice). Means without a common letter differ, P < 0.05.

Fig 3: DDR-related cytokines derived from glioma cells are synergistic with DDR alterations in vitro and in vivo.(a) Gene expression profiling revealed the associations between DDR-related cytokines and the DDR scores or DDR genes. (b) IHC assays were used to detect the expression level of six cytokines, C5, SAA1, MDK, IL6, VEGFA and TNFSF4 in glioma samples within the different DDR clusters (20×). (c) Western blot validation of DDR-associated protein and cytokine expression levels in LN229, LN229R, HG7, HG7R, GL261 and GL261R cells. (d) ELISA assay revealed differential expression of six cytokines in the medium of LN229, LN229R, HG7, HG7R, GL261 and GL261R cells. (e) Cytokine expression in xenograft gliomas formed by GL261 and GL261R cells was detected by IHC assays (20×).

Fig 4: Assessment of mRNA expression levels in liver tissues presented as mean differences (MD) between the infected and control groups (n = 15 mice per group): Saa1 (MD 6.40 ± 1.97, 95%CI 2.37; 10.43; P = 0.0031), Saa2 (MD 9.58 ± 4.90; 95%CI - 0.50; 19.66: P = 0.0615), Saa3 (MD 0.47 ± 1.06; 95%CI - 1.70; 2.63; P = 0.6618) and Tnf (MD 1.68 ± 0.64; 95%CI 0.35; 3.00; P = 0.0160).