Fig 1: Asthma-induced ferroptosis. (a) H&E staining was used for the detection of the injury of lung tissues. (b) Perls staining was used for the detection of iron in lung tissues. (c–e) The levels of free iron ions were detected with commercial kits. (f, g) The expression of ferroprotein, including FTL and FTH1, was detected with ELISA. ***p < 0.001. FTL: ferritin light chain; FTH1: ferritin heavy chain.
Fig 2: Ferr-1 and 3-MA relieved the accumulation of free iron ions in lung tissues in asthma mice. (a) The levels of FTL in BALF were determined with ELISA. (b) The levels of FTH1 in BALF were determined with ELISA. (c–e) The levels of free iron ions in BALF and lung tissues were detected with commercial kits. (f, g) Perls staining was used for the detection of Fe in lung tissues. ***p < 0.001 vs. the control; #p < 0.05, ##p < 0.01, and ###p < 0.001 vs. the model. Ferr-1: ferrostatin-1; 3-MA: 3-methyladenine; FTL: ferritin light chain; FTH1: ferritin heavy chain; BALF: bronchoalveolar lavage fluid.
Supplier Page from Abcam for Mouse Ferritin ELISA Kit (FTL)