Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of human Heat Shock 70kDa Protein 1A (also known as HSPA1A) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human HSPA1A antibody. HSPA1A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human HSPA1A Antibody is added and binds to HSPA1A in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated HSPA1A antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human HSPA1A. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm