Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of Mouse Macrophage Migration Inhibitory Factor (also known as MIF) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse MIF antibody. MIF present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse MIF Antibody is added and binds to MIF in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated MIF antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse MIF. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm