Description
Principle of the assay: human OPG ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for OPG has been precoated onto 96-well plates. Standards (NSO,E22-L401) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for OPG is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human OPG amount of sample captured in plate.
Background: Osteoprotegerin (OPG) is identical to osteoclastogenesis inhibitory factor (OCIF), a soluble member of the tumor-necrosis factor receptor family that inhibits osteoclastogenesis.1 OPG is considered to play an important rolein the regulation of bone resorption by modifying osteoclast differentiation.2 Osteoprotegerin is an independent riskfactor for the progression of atherosclerosis and onset of cardiovascular disease.3 It can act as a soluble factor inthe regulation of bone mass and imply a utility for OPG in the treatment of osteoporosis associated with increased osteoclast activity.4 OPG system may play a critical role in the development of osteolytic bone disease in multiplemyeloma and that targeting this system may have therapeutic potential.5 OPG also plays a role in the vascularsystem.6 The standard product used in this kit is recombinant OPG, a 55KDa glycoprotein