Description
Provides rapid purification and concentration of total RNA from a variety of samples (e.g., LCM tissues, fine needle aspirates, and FACS-sorted cells)
A simple and reliable technology for everyday RNA purificationPureLink™ technology combines guanidine-isothiocyanate lysis with the speed, purity, and ease-of-use of silica-membrane purification. The safe and easy procedure can be completed in typically less than 15 minutes with >85% recovery from the first elution. The kit can be used for clean-up of purified RNA from enzymatic reactions, desalting of RNA samples after phenol extraction or ethanol precipitation, and concentration of RNA. Purify RNA from up to 10mg of tissue or 10
5 cells. Clean up and concentrate RNA amounts ranging from pg to 100µg in volumes as small as 10µL. Purified RNA is ready for use in downstream applications such as RT-PCR, qRT-PCR, northern blots, cDNA library construction, and nuclease protection assays.
A simple and rapid procedureHomogenize your sample in lysis buffer, add 70% ethanol, and mix. Deposit the resulting solution into a PureLink Micro kit column with collection tube, and centrifuge for one minute. Nucleic acids bind to the PureLink silica membrane in the column, and after subsequent washes and on-column digestion with DNase, purified RNA can be eluted with RNase-free water.
- Effective: Expect excellent RNA recovery (up to 100µg total RNA) from small samples
- Convenient and safe: The extraction procedure uses no phenol/chloroform, CsCl gradients, or precipitation with either LiCl or ethanol
- Fast: Obtain high-quality total RNA, typically in just 15 minutes
- Reliable: Obtain ready-to-use RNA suitable for any downstream application