Human Interleukin 12,IL-12/P40 ELISA KIT CSB-E04598h from CUSABIO Technology LLC

Product Specs
ItemHuman Interleukin 12,IL-12/P40 ELISA KIT
CompanyCUSABIO Technology LLC
Price $495.00Supplier Page View Company Product Page
Catalog NumberCSB-E04598h
ApplicationsELISA
MethodELISA Kit
Research AreaImmunology
NCBI Gene AliasesCLMF, CLMF2, IL-12B, IMD28, IMD29, NKSF, NKSF2
NCBI Full Gene Nameinterleukin 12B
Assay Time1-5h
Volume50-100ul
Sensitivity0.039 ng/mL
Sample Typeserum, plasma, tissue homogenates
PrecisionIntra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Absorbance / Excitation450 nm
Detection Range0.156 ng/ml-10 ng/ml
Quantity96T

Specifications/Features

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL12B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL12B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL12B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL12B bound in the initial step. The color development is stopped and the intensity of the color is measured.

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