Fig 1: The gene expressions of DLL3, IL-33, LG3BP (also LGASLS3BP), and HSPA8, and their associations with overall survival were analysed using a UCSC Xena Functional Genomics Explorer. (A) Heat maps of DLL3, IL-33, LG3BP, and HSPA8 expressions in patients with stomach cancer and normal persons. This data set shows the gene-level transcription estimates, as in log2(x + 1) transformed RSEM normalized count. The default view was set to center each gene or exon to zero by independently subtracting the mean of each gene or exon on the fly. (B) Kaplan–Meier plot is displayed to compare the 5-year survival rate between patients with high target gene expression and low expression. DLL3, delta-like ligand 3; IL-33, interleukin-33; LG3BP, galectin-3–binding protein; HSPA8, heat shock cognate 71 kDa protein; RSEM, RNA-seq by expectation maximization.
Fig 2: Differences in the distribution of serum IL-33 levels in each study group. a Box-scatter plot showing differences in IL-33 serum levels in healthy controls (n = 73) and overweight/obese subjects (n = 144) by box-scatter plot; Student’s t test were performed in two groups. b Violin plot showing basal serum IL-33 levels in each study group by sex; 2-way ANOVA analysis were performed to analyze the effect of group and sex on IL-33 level
Fig 3: Protein levels of IL-33 (A), IL-25 (B), TSLP (C), and PAF (D) in plasma samples of non-celiac subjects (control) (n = 8), de novo diagnosed CD patients (de novo) (n = 6), and CD patients on GFD (GFD) (n = 7). Scatter plots show mean ± SD. *p-value <0.05.
Fig 4: Relationship between IL-33 and major clinic-metabolic parameters in MHOO and MUOO group. Coefficients (r) and P values were calculated by the Spearman’s correlation model. The correlation level was considered significant when < 0.05
Fig 5: The expression of IL-33 in DLL3-overexpressed MKN45 cells. (A) The mRNA expression of IL-33 in DLL3-overexpressed and DLL3–downregulated MKN45 cells was detected by qPCR. The relative expression level of mRNA was normalized to β-actin expression. MKN45, MKN45 cells; DLL3–MKN45, DLL3-overexpressed MKN45 cells; siRNA–DLL3–MKN45, DLL3-downregulated MKN45 cells. (B) The expression of IL-33 in the supernatant was detected by ELISA after co-culture of macrophages and DLL3-overexpressed MKN45 cells. Control, RPMI 1640 medium; MKN45, supernatant from MKN45 cells; DLL3–MKN45, supernatant from DLL3-overexpressed MKN45 cells; Mφ, supernatant from macrophages; Mφ + MKN45, supernatant from co-culture of macrophages and MKN45; Mφ + DLL3–MKN45, supernatant from co-culture of macrophages and DLL3-overexpressed MKN45 cells. Error bars represent standard error of means from three independent experiments. Statistical significance is indicated by *P < 0.05 and **P < 0.01. IL, interleukin; DLL3, delta-like ligand 3; ELISA, enzyme-linked immunosorbent assay.
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