Fig 1: ROC curve analysis in the diagnosis of all stages and early-stage GC. (A) The performance of the EFNA1, MMP13 and combination of EFNA1 and MMP13 in distinguishing all stages and early-stage ESCC from normal controls are shown for the test cohort. (B) The performance of the EFNA1, MMP13 and combination of EFNA1 and MMP13 in distinguishing all stages and early-stage GC from normal controls are shown for the validation cohort. The area under the black line is 0.5, for reference. ROC Receiver operating characteristic, GC gastric cancer.
Fig 2: The concentration of serum EFNA1 and MMP13 shown in scatter plots and violin plots. (A) OD values of EFNA1 and MMP13 from GC sera (115) and normal sera (123) in the test cohort. (B) OD values of EFNA1 and MMP13 from GC sera (95) and normal sera (100) in the validation cohort. GC gastric cancer, ELISA enzyme-linked immunosorbent assay.
Fig 3: Serum levels of EFNA1 and MMP13 in ESCC patients before and after surgery. (A, C) Serum levels of EFNA1 in ESCC patients before and after surgery. (B, D) Serum levels of MMP13 in ESCC patients before and after surgery. The black horizontal line in the box plot is the mean and the error line is SE. Abbreviation: ESCC: esophageal squamous cell carcinoma.
Fig 4: Screening of secreted protein encoded by SE-driven genes in ESCC. A. The Venn diagram show up-regulated SEs-driven genes in ESCC. SE-associated genes in KYSE510, TE7 and TE7&KYSE510 cells (orange circles), Up-regulated genes in ESCC (grey circles) and their combinations (overlapping regions). B. H3K27ac ChIP-Seq binding profiles of representative up-regulated super enhance-driven genes in ESCC lines. C. mRNA Expression levels of EFNA1and MMP13 in various tumour cell lines (Red is the expression in esophageal cancer). D. Scatter plot of mRNA Expression levels of EFNA1and MMP13 in ESCC tissues (GSE53624). The black horizontal line in the box plot is the mean and the error line is SE. E. Survival curve analysis of mRNA expression levels of EFNA1and MMP13 in ESCC tissues (GSE53624). F. Expression of serum EFNA1 and MMP13 in small samples (33 ESCC patients vs 36 normal).
Fig 5: ROC curve for iFBPanel in all stages and early-stage of ESCC vs. normal control. A. The performance of the iFBPanel in distinguishing all stages and early-stage ESCC from normal controls are shown for the test cohort. B. The performance of the iFBPanel in distinguishing all stages and early-stage ESCC from normal controls are shown for the validation cohort. The area under the black line is 0.5. Abbreviation: ROC: Receiver operating characteristic; iFBPanel: EFNA1+ MMP13+ CEA+ Cyfra21-1+ SCCA; ESCC: esophageal squamous cell carcinoma.
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