Fig 2: Expression of human leukocyte antigen E [low expression (A); high expression (B)] and NK cells(the NK cells exhibit CD3 negative and CD56 positive staining (C), whereas CD56 negative and CD3 positive (D) or CD56 positive and CD3 positive (E) are not associated with NK cells) in ESCC [positive (F) negative (G)].

Fig 3: Distinct tumor immune microenvironment of patients with EGFR-TKI-resistant NSCLC with LSM-high(A and B) Representative micrographs for hematoxylin-eosin and fluorescence immunohistochemistry assay for CD20, CD3, pan-cytokeratin (CK), CD4, CD8, and CD56 for a patient with LSM-low (A) and LSM-high (B). All microscopy images include scale bars of 100 μm and 50 μm, as indicated.(C–H) Boxplots comparing the distribution of positive cell/mm2 between LSM-low (n = 6) and LSM-high (n = 10) subgroups for CD8 (C), CD4 (D), CD3 (E), CD20 (F), CD56 (G), and pan-CK (H) in patients with EGFR-TKI-resistant NSCLC. Statistical comparisons were performed using t test. The box represents the interquartile range, with the line inside indicating the median value. Each dot marks the individual data point, and dots located outside the whiskers represent outliers. The top and bottom edges of the box correspond to the lower and upper quartiles, while the whiskers extending from the box show the minimum and maximum values in the dataset.