Cell Cycle Assay Kit (Fluorometric - Green) ab112116 from Abcam

Product Specs
ItemCell Cycle Assay Kit (Fluorometric - Green)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab112116
Quantity100 Test
Sample TypeAdherent cells, Suspension cells
MethodFlow Cyt, FuncS

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Website: http://www.abcam.com

(1) Inhibition of FOS‐Like Antigen 1 Reduces Chemoresistance to Temozolomide Through Stemness Reprogramming via IL‐6/STAT3 Tyr705 PathwayMedComm (2020)February 1, 2026JeongMin Sim, JeongMan Park, JinHyung Heo, Yu Jin Kim, Dongkil Kim, Yong Hyun Jeon, Jae‐Eon Lee, Seon Hee Choi, Hak Jin Kim, So Jung Hwang, et al.and siRNA‐treated cells (5 × 105 cells/mL) were collected and incubated with Nuclear Green CCS1 dye (#ab112116, Abcam) in 0.5 mL of Fluorometric‐Green cell cycle buffer. The cells were maintained at 37°C for 1 h

(2) KIFC1 Overexpression Promotes Pancreatic Carcinoma Progression via Stabilising BUB1BJ Cell Mol MedAugust 26, 2025Ao Cui, Ying‐Xue Yu, Mei‐Xue Xiong, Ji‐Yang Wang, Ye‐Qing Zou, Ya‐Qiong Zhu, Long‐Jian Ran, Yu Zhang, Rui‐Xiang Liu, Ming‐Yi Dong, et al.overnight. The following day, the cells were washed twice with PBS, stained with 0.5 mL of PI/RNase (Abcam, ab112116, USA) and resuspended. The cells were incubated for 30 min at room temperature in the dark. Afterwards,

(3) Metabolomics analysis reveals cytotoxic effects of ouabain towards psoriatic keratinocytes via impairment of glutathione metabolismMolecular genetics and genomics : MGGMay 1, 2023Zhou X, Fei F, Song W, Ma H, Xu Z, Yue J, Cao B, Sun R, Zhao Y, Yang Y, et al.of cells that were in G0/G1, S and G2/M phases were determined by flow cytometry using an assay kit (ab112116, Abcam, MA, USA) according to the instructions provided. Untargeted GC/MS metabolomics analysis Cell

(4) Pepsin enhances glycolysis to promote malignant transformation of vocal fold leukoplakia epithelial cells with dysplasiaEuropean Archives of Oto-Rhino-LaryngologyApril 1, 2023Haitong Li, Shasha Zhang, Shuihong Zhou, Yangyang Bao, Xiaojuan Cao, Lifang Shen, Bin Xu, Weimin Gao, Yunzhen Luoand centrifuged at 1000 rpm for 5 min. Cell cycle analysis was performed using the Cell Cycle kit (ab112116, Abcam, Cambridge, UK) and Apoptosis Analysis Kit (Yeasen, 40301ES50, Shanghai, CN). Cells were resuspended

(5) SUMO Proteomics Analyses Identify Protein Inhibitor of Activated STAT-Mediated Regulatory Networks Involved in Cell Cycle and Cell ProliferationJournal of Proteome ResearchMarch 3, 2023Chongyang Li, Alison Boutet, Cristina Mirela Pascariu, Trent Nelson, Mathieu Courcelles, Zhaoguan Wu, Simon Comtois-Marotte, Gregory Emery, Pierre ThibaultPBS. After all washes, cells were resuspended in PBS and processed with the cell cycle kit (Abcam, ab112116). Cellular DNA content was determined by flow cytometry with the BD FACSCanto II system and BD FACS

(6) CENPF/CDK1 signaling pathway enhances the progression of adrenocortical carcinoma by regulating the G2/M-phase cell cycleJournal of Translational MedicineFebruary 5, 2022Yu-gang Huang, Dan Li, Li Wang, Xiao-min Su, Xian-bin Tanganalyzed using FlowJo7.6 software. Cell cycle assay For cell cycle assay, a cell cycle assay kit (ab112116, Abcam) was purchased. After being transfected with siCENPF, CDK1, or siNC for 48 h, SW13 cells were

(7) EMS1/DLL4-Notch Signaling Axis Augments Cell Cycle-Mediated Tumorigenesis and Progress in Human Adrenocortical CarcinomaFrontiers in OncologyNovember 10, 2021Yu-Gang Huang, Ya Wang, Rui-Juan Zhu, Kai Tang, Xian-Bin Tang, Xiao-Min Suapoptosis. Data were analyzed using FlowJo7.6 software. 2.8.8 Cell Cycle Assay Cell cycle assay kit (ab112116, Abcam, Cambridge, MA, USA) was utilized for cell cycle assay. After transfection with siESM1 or siNC

(8) Characterizing the Low-Dose Effects of Methylmercury on the Early Stages of Embryo Development Using Cultured Human Embryonic Stem CellsEnvironmental health perspectivesJuly 1, 2021Li B, Qiao C, Jin X, Chan HM.resuspended and stained in the nuclear green CCS1 solution provided in the Cell Cycle Assay kit (Catalog no. ab112116; Abcam Inc.). After incubating for 40 min at 37°C, fluorescent intensity was quantified using a BD LSR

(9) Hsa_circ_0085576 promotes clear cell renal cell carcinoma tumorigenesis and metastasis through the miR-498/YAP1 axisAgingJune 15, 2020Liu G, Zhou J, Piao Y, Zhao X, Zuo Y, Ji Z.cycle analysis were carried out following the manufacturer’s instructions of Cell Cycle Assay Kit (ab112116, Abcam). Attached cells were harvested cells and fixed in 70% ice-cold ethanol overnight at 4 °C. Finally,

(10) An Aurora Kinase B-Based Mouse System to Efficiently Identify and Analyze Proliferating CardiomyocytesFrontiers in cell and developmental biologyJanuary 1, 2020Fu W, Liao Q, Li L, Shi Y, Zeng A, Zeng C, Wang WE.fluorescence-activated cell sorting and cell cycle analysis using a Cell Cycle Assay Kit (Fluorometric-Green, Abcam, ab112116) according to the manufacturer’s instructions. The cardiomyocytes were prepared at a density of 1 ×

(11) Citron Rho-Interacting Serine/Threonine Kinase Promotes HIF1a-CypA Signaling and Growth of Human Pancreatic AdenocarcinomaBioMed research internationalJanuary 1, 2020Cong L, Bai Z, Du Y, Cheng Y.percentage of apoptotic cells was calculated. The cell cycle was analyzed with Cell Cycle Assay Kit (Abcam, ab112116) by flow cytometry. Ki67 expression was analyzed with an APC-conjugated anti-Ki67 antibody (ThermoFisher,

(12) Circular RNA CEP128 acts as a sponge of miR-145-5p in promoting the bladder cancer progression via regulating SOX11Molecular MedicineDecember 1, 2018Zhun Wu, Wei Huang, Xuegang Wang, Tao Wang, Yuedong Chen, Bin Chen, Rongfu Liu, Peide Bai, Jinchun XingFlow cytometry (FCM) assay Transfected cells were subjected to PI staining for detection with Cell Cycle assay Kit (ab112116, Abcam). Then they were subjected to FITC-Annexin V and PI double staining for flow cytometry detection

(13) MicroRNA-143 suppresses oral squamous cell carcinoma cell growth, invasion and glucose metabolism through targeting hexokinase 2Bioscience reportsJune 30, 2017Sun X, Zhang L.analyzed. Cell cycle analysis The cell cycle was analyzed using the Cell Cycle Assay Kit (Abcam, #ab112116) according to the manufacturer’s protocol. 2 c 2017 The Author(s). This is an open access article

(14) MicroRNA-497 suppresses osteosarcoma tumor growth in vitro and in vivoOncology lettersMarch 1, 2016Ge L, Zheng B, Li M, Niu L, Li Z.Cell cycle progression was monitored using Cell Cycle Assay Kit (Fluorometric ‑ Green) (catalogue number, ab112116; Abcam, Cambridge, MA, USA). The cells were acquired using BD™ LSR II flow cytometer (BD Biosciences,

(15) Restoration of WNT4 inhibits cell growth in leukemia-derived cell linesBMC cancerNovember 25, 2013García-Castro B, Alvarez-Zavala M, Riveros-Magaña AR, Ortíz-Lazareno PC, Ratkovich-González S, Hernández-Flores G, Bravo-Cuellar A, Jave-Suarez LF, Aguilar-Lemarroy A.340242; Becton, Dickinson and Company), and the Cell Cycle Assay kit (Fluorometric-Green, cat. no. ab112116, Abcam). Both were used following the manufacturer’s instructions. DNA QC particles (cat. no. 349523)

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Cell Cycle Assay Kit (Fluorometric - Green) from Abcam

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Cell Cycle Assay Kit (Fluorometric - Green) from Abcam

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