Complex I Enzyme Activity Dipstick Assay Kit ab109720 from Abcam

Product Specs
ItemComplex I Enzyme Activity Dipstick Assay Kit
CompanyAbcam
Price $369.00Supplier Page View Company Product Page
Catalog Numberab109720
Quantity48 Test
ApplicationsFuncS
MethodFuncS
Research AreaMetabolism
NCBI Full Gene NameNADH:ubiquinone oxidoreductase complex assembly factor 1
NCBI Gene AliasesCGI-65, CGI65, CIA30, MC1DN11

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+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Electron Leak From the Mitochondrial Electron Transport Chain Complex I at Site I(Q) Is Crucial for Oxygen Sensing in Rabbit and Human Ductus ArteriosusJournal of the American Heart AssociationJuly 4, 2023Read AD, Bentley RET, Martin AY, Mewburn JD, Alizadeh E, Wu D, Lima PDA, Dunham-Snary KJ, Thébaud B, Sharp W, et al.Measurements Complex I activity was quantified using the Complex I Enzyme Activity Dipstick Assay (ab109720;Abcam). rbDASMCs were collected 3 hours after incubation with 50 μmol/L S1QEL or vehicle (DMSO). After

(2) Alpha-lipoic acid supplementation corrects pathological alterations in cellular models of pantothenate kinase-associated neurodegeneration with residual PANK2 expression levelsOrphanet Journal of Rare DiseasesApril 12, 2023Marta Talaverón-Rey, Mónica Álvarez-Córdoba, Irene Villalón-García, Suleva Povea-Cabello, Juan M. Suárez-Rivero, David Gómez-Fernández, Ana Romero-González, Alejandra Suárez-Carrillo, Manuel Munuera-Cabeza, Paula Cilleros-Holgado, et al.activity Complex I activity in whole cells was measured using the Complex I Enzyme Activity Dipstick Assay Kit (ab109720, ABCAM, Cambridge, MA, USA) according to manufacturer’s instructions. Three biological replicates were

(3) Transgenic NADH dehydrogenase restores oxygen regulation of breathing in mitochondrial complex I-deficient miceNature communicationsMarch 1, 2023Jiménez-Gómez B, Ortega-Sáenz P, Gao L, González-Rodríguez P, García-Flores P, Chandel N, López-Barneo J.activity in the SCG and kidney was determined using complex I enzyme activity dipstick assay kit (ref. ab109720, Abcam) following the manufacturer’s instruction with minor modifications7. For each replicate, SCGs

(4) Biallelic pathogenic variants in COX11 are associated with an infantile‐onset mitochondrial encephalopathyHuman MutationDecember 1, 2022Rocio Rius, Neal K. Bennett, Kaustuv Bhattacharya, Lisa G. Riley, Zafer Yüksel, Luke E. Formosa, Alison G. Compton, Russell C. Dale, Mark J. Cowley, Velimir Gayevskiy, et al.activities of Complex I and IV in fibroblasts were determined using dipstick activity assays (Abcam #ab109720; #ab109876), and showed a mild decrease in CIV (decreased by 40% relative to control A), but no difference

(5) SARS-CoV-2 mitochondriopathy in COVID-19 pneumonia exacerbates hypoxemiaRedox biologyDecember 1, 2022Archer SL, Dasgupta A, Chen KH, Wu D, Baid K, Mamatis JE, Gonzalez V, Read A, Bentley RE, Martin AY, et al.complex I activity Complex I activity was quantified using the Complex I Enzyme Activity Dipstick Assay (ab109720, Abcam, Toronto, ON). BEAS-2B cells were collected either 48 hours post HCoV-OC43 infection or 96 hours

(6) Therapeutic approach with commercial supplements for pantothenate kinase-associated neurodegeneration with residual PANK2 expression levelsOrphanet Journal of Rare DiseasesAugust 9, 2022Mónica Álvarez-Córdoba et al.activity Complex I activity in whole cells was measured using the Complex I Enzyme Activity Dipstick Assay Kit (ab109720, ABCAM, Cambridge, MA, USA) according to manufacturer’s instructions. Three biological replicates were

(7) Down regulation of the expression of mitochondrial phosphopantetheinyl-proteins in pantothenate kinase-associated neurodegeneration: pathophysiological consequences and therapeutic perspectivesOrphanet Journal of Rare DiseasesDecember 1, 2021Mónica Álvarez-Córdoba, Marta Talaverón-Rey, Irene Villalón-García, Suleva Povea-Cabello, Juan M. Suárez-Rivero, Alejandra Suárez-Carrillo, Manuel Munuera-Cabeza, Joaquín J. Salas, José A. Sánchez-Alcázaractivity Complex I activity in whole cells was measured using the Complex I Enzyme Activity Dipstick Assay Kit (ab109720, ABCAM, Cambridge, MA, USA) according to manufacturer’s instructions. Three biological replicates were

(8) Irp2 regulates insulin production through iron-mediated Cdkal1-catalyzed tRNA modificationNature communicationsJanuary 15, 2020Santos MCFD, Anderson CP, Neschen S, Zumbrennen-Bullough KB, Romney SJ, Kahle-Stephan M, Rathkolb B, Gailus-Durner V, Fuchs H, Wolf E, et al.measured in INS-1 832/13 cell extracts using Complex I Enzyme Activity Dipstick Assay Kit (Abcam, cat# ab109720) and Complex IV Rodent Enzyme Microplate Assay Kit (Abcam, cat# ab109911) following the manufacturer’s

(9) Transcriptomic Signature of Right Ventricular Failure in Experimental Pulmonary Arterial Hypertension: Deep Sequencing Demonstrates Mitochondrial, Fibrotic, Inflammatory and Angiogenic AbnormalitiesInternational Journal of Molecular SciencesSeptember 12, 2018Francois Potus, Charles Colin Thomas Hindmarch, Kimberly J. Dunham-Snary, Jeff Stafford, Stephen L. Archer

(10) Regulation of energy metabolism during early mammalian development: TEAD4 controls mitochondrial transcriptionDevelopmentJanuary 1, 2018Ram P. Kumar et al.assay Complex I and IV activity were determined using the Complex I Enzyme Activity Dipstick Assay Kit (Abcam, ab109720) and Complex IV Rodent Enzyme Activity Dipstick Assay Kit (Abcam, ab109878), respectively, according

(11) Migration and invasion of drug-resistant lung adenocarcinoma cells are dependent on mitochondrial activityExperimental & Molecular MedicineDecember 1, 2016Ji Hoon Jeon et al.enzyme activity Complex 1 activity was quantified by a complex 1 enzyme activity dipstick assay kit (ab109720, Abcam) as per the manufacturer's instructions. Briefly, lysates from 1 × 105–4 × 106 of A549 and A549R

(12) Natural Product Screening Reveals Naphthoquinone Complex I Bypass FactorsPLOS ONESeptember 13, 2016Scott B. Vafai, Emily Mevers, Kathleen W. Higgins, Yevgenia Fomina, Jianming Zhang, Anna Mandinova, David Newman, Stanley Y. Shaw, Jon Clardy, Vamsi K. Moothaactivities of OXPHOS complex I and IV were determined using commercial dipstick assays from Abcam (ab109720 for complex I and ab109878 for complex IV), following the manufacturer’s protocols. Assays for complex

(13) Promotion of mitochondrial biogenesis by necdin protects neurons against mitochondrial insultsNature communicationsMarch 14, 2016Hasegawa K, Yasuda T, Shiraishi C, Fujiwara K, Przedborski S, Mochizuki H, Yoshikawa K.with immobilized anti-Complex I antibodies combined with in-gel activity measurement (dipstick assay, ab109720, Abcam). Cell lysates (30 μg protein) were used for the assay according to the manufacturer's protocol.

(14) Pyruvate carboxylation enables growth of SDH-deficient cells by supporting aspartate biosynthesisNature cell biologyOctober 1, 2015Cardaci S, Zheng L, MacKay G, van den Broek NJ, MacKenzie ED, Nixon C, Stevenson D, Tumanov S, Bulusu V, Kamphorst JJ, et al.proteins by immunoblotting and to assess the activity of Complex I by the dipstick assay kit (Abcam, ab109720), according to manufacturer instructions. Immunoblotting Cells were washed twice with cold PBS and

(15) Mitochondrial abnormalities in temporal lobe of autistic brainNeurobiology of diseaseJune 1, 2013Tang G, Gutierrez Rios P, Kuo SH, Akman HO, Rosoklija G, Tanji K, Dwork A, Schon EA, Dimauro S, Goldman J, et al.Mitochondrial Dipstick Assay kit, performed according to the manufacturer’s instructions (Abcam ab109876, ab109720). Fifteen micrograms of proteins was allowed to wick up laterally through the dipstick membrane, the

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Complex I Enzyme Activity Dipstick Assay Kit from Abcam

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Complex I Enzyme Activity Dipstick Assay Kit from Abcam

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