Fig 1: TNF-alpha secretion by U-2 OS cells grown for 1, 3 and 7 days on bare Ti-6Al-4V samples (Ti-6Al-4V), on samples coated with as-synthesized silicalite-1 films (SF-RT) or with silicalite-1 films heated up to 500 °C (SF-500), and on the control microscopic glass coverslips (GS). NCS – neocarzinostatin treatment (700 ng/mL for 1 hour), a positive control.
Fig 2: The molecular function and mechanism of miR-27a-3p in human RASFs. Upregulating miR-27a-3p inhibited TLR5 expression to attenuate proliferation and pro-inflammatory cytokines secretion, as well as to promote apoptosis. miR, microRNA; RASFs, RA synovial fibroblasts; TLR5, toll-like receptor 5; IL, interleukin; TNF, tumor necrosis factor.
Fig 3: Role of miR-27a-3p in the proliferation, apoptosis and inflammation of RASFs. miR-27a-3p was exogenously overexpressed in RASFs by transfecting miR-27a-3p mimic (miR-27a-3p). (A) Reverse transcription-quantitative polymerase chain reaction assay detected the miR-27a-3p level following transfection. (B and C) Cell proliferation and apoptosis were investigated using MTT assay and flow cytometry. (D-F) Products of IL-6, IL-1ß and TNF-a were determined by ELISA kits. *P<0.05, compared with miR-NC mimic (miR-NC). miR, microRNA; RASFs, RA synovial fibroblasts; IL, interleukin; TNF, tumor necrosis factor; NC, negative control.
Fig 4: Effect of TLR5 on the proliferation, apoptosis and inflammation in RASFs. TLR5 was exogenously silenced in RASFs by transfecting siRNA targeting TLR5 (si-TLR5). (A) Western blotting detected the TLR5 level. (B and C) Cell proliferation and apoptosis were investigated using MTT assay and flow cytometry. (D-F) Products of IL-6, IL-1ß and TNF-a were determined by ELISA kits. *P<0.05, compared with scrambled siRNA (si-NC). TLR5, toll-like receptor 5; RASFs, RA synovial fibroblasts; NC, negative control; IL, interleukin; TNF, tumor necrosis factor. TLR5, toll-like receptor 5; RASFs, RA synovial fibroblasts; IL, interleukin; TNF, tumor necrosis factor; NC, negative control.
Fig 5: Influence of TLR5 overexpression on miR-27a-3p-induced effects in RASFs. RASFs were co-transfected with miR-27a-3p and pcDNA-TLR5 (TLR5) vector or pcDNA vector. (A) Western blotting detected the TLR5 level. (B and C) Cell proliferation and apoptosis were investigated following transfection using MTT assay and flow cytometry. (D-F) Products of IL-6, IL-1ß and TNF-a were determined by ELISA kits. *P<0.05, compared with miR-NC or miR-27a-3p+pcDNA. TLR5, toll-like receptor 5; miR, microRNA; RASFs, RA synovial fibroblasts; IL, interleukin; TNF, tumor necrosis factor; NC, negative control.
Supplier Page from Abcam for Human TNF alpha ELISA Kit