NOS Non-Enzymatic Assay Kit from Eagle Bioscience

In aqueous solution, nitric oxide rapidly degrades to nitrate and nitrite. Spectrophotometric quantitation of nitrite using Griess Reagent is straightforward, but does not measure nitrate. This kit employs metallic cadmium for quantitative conversion of nitrate to nitrite (figure 1) prior to quantitation of nitrate using Griess reagent — thus providing for accurate determination of total NO production