Fig 1: Expression of SPP1 protein in (A) callus, (B) heterotopic ossification tissues and (C) plasma from patients. Western blotting was used to determine protein expression in callus and heterotopic ossification tissues and ELISA was performed to measure protein content in plasma. SPP1, osteopontin; TBI, traumatic brain injury *P<0.05, **P<0.01 vs. control group.
Fig 2: Expression of SPP1 mRNA in (A) callus, (B) heterotopic ossification tissues and (C) plasma from patients. Reverse transcription-quantitative PCR was used to determine mRNA expression levels. SPP1, osteopontin; TBI, traumatic brain injury. *P<0.05, **P<0.01 vs. control group.
Fig 3: Parameters to assess the degree hepatic fibrosis.Blood platelets count (A), serum type IV collagen (B), serum hyaluronic acid (C), and serum OPN (D) levels in F0, F1, F2, F3, and F4 group of patients with HCV infection. Among the 4 biomarkers depicted to assess the stage of hepatic fibrosis, only serum OPN demonstrated a sequential increase from F0 through F4 with a significant difference (P < 0.001) between each group that coincides with the degree of fibrosis.
Fig 4: Effect of miR-433 overexpression on the expression of SPP1. (A) Bioinformatics prediction of direct interaction between miR-433 and the 3'-untranslated region of SPP1 mRNA. miRanda (http://www.microma.org/rnicroma/home.do) was used to predict genes that might regulate SPP1. (B) Identification of the interaction between miR-433 and SPP1 mRNA by dual luciferase reporter assay. (C) Expression of miR-433 in hFOB1.19 cells transfected with agomiR-433 or agomiR-NC. (D) Expression of SPP1 mRNA in hFOB1.19 cells transfected with agomiR-433 or agomiR-NC. (E) Expression of SPP1 protein in hFOB1.19 cells transfected with agomiR-433 or agomiR-NC. SPP1, osteopontin; miR, microRNA; NC, negative control; TBI, traumatic brain injury. *P<0.05 **P<0.01 compared with the agomiR-NC group.
Fig 5: Receiver Operating Characteristic (ROC) curve analysis to evaluate the competence of serum OPN as a diagnostic marker for the degree of hepatic fibrosis in patients with HCV infection.(A) ROC curves of serum OPN, platelet count, HA, and collagen type IV for discriminating F1 against F2/F3/F4. (B) ROC curves of serum OPN, platelet count, HA, and collagen type IV for discriminating F1/F2 against F3/F4. (C) ROC curves of serum OPN, platelet count, HA, and collagen type IV for discriminating F1/F2/F3 against F4. OPN, osteopontin; HA, hyaluronic acid; F, fibrosis.
Supplier Page from Abcam for Human Osteopontin ELISA Kit