Fig 1: IGF1R downregulation mediates VSMC apoptosis by promoting AKT expression. (A) The expression of IGF1R protein in TAD aortic wall tissue and respective adjacent CON tissues were analyzed using IHC. (B) IGF1R expression scores are shown as box plots. (** p < 0.001). (C) Tunel stain showed PPP-induced apoptosis of NSMC. (D) STRING database shows IGF1R interaction with AKT. (E) Western blot showed that PPP-induced downregulation of IGF1R expression in NSMC further leads to the increased expression of AKT and Caspase3. (F) Receiver operating characteristic analysis of the sensitivity and specificity of the predictive value of the IGF1R in GSE52093. (G) The serum concentration of IGF1R levels between the TAD and normal patients in our data (**** p = 0.000). (H) Receiver operating characteristic analysis of the sensitivity and specificity of the predictive value of the serum concentration of IGF1R levels in our data.
Fig 2: Signal axis screening and specimen verification. (A) Sankey diagram for the circRNA-microRNA-mRNA-pathways axis screened out in this study. Each rectangle represents a gene or pathway, and the connection degree of each gene is visualized based on the size of the rectangle. (B–D) The expression levels of the hsa-cic-0007386/miR-1271-5P/IGF1R between the TAD and CON group in the GSE97745, GSE98770, and GSE52093 datasets, respectively. * p < 0.05, ** p < 0.01. (E–G) The expression levels of the hsa-cic-0007386/miR-1271-5P/IGF1R between the TAD and CON group in our aortic dissection specimen. * p < 0.05, ** p < 0.01.
Fig 3: Prediction and validation of Let-7g-5p potential target genes and the effects on SUNE1 cells. (A) Searching for the target gene of Let-7g-5p in the database TargetScan, miRDB, TargetMiner. (B) Dual-luciferase reporter assay was used to verify that Let-7g-5p directly regulated IGF-1R expression by 3′UTR. (C–E) RT-qPCR and western blot were used to detect the expression levels of IGF-1R mRNA and protein in the 5 groups. (F) CCK-8 assay was used to detect the cell viability in the 5 groups. * P<0.05, ** P<0.01, versus control group; # P<0.05, ## P<0.01, versus Let-7g-5p mimics group; ^ P<0.05, ^^ P<0.01, versus IGF-1R group. RT-qPCR – real-time quantitative polymerase chain reaction; CCK-8 – cell counting kit-8.
Fig 4: Effects of IGF-1R overexpression on the inhibition of migration and invasion by Let-7g-5p. (A–D) The percentage of wound width and migration rate of SUNE1 cells in the five groups were detected using scratch assay and Transwell assay, respectively. * P<0.05, ** P<0.01, versus control group; # P<0.05, ## P<0.01, versus Let-7g-5p mimics group; ^ P<0.05, ^^ P<0.01, versus IGF-1R group.
Fig 5: Effects of IGF-1R and Let-7g-5p on EMT. (A–G) expression levels of E-cadherin, vimentin, and MMP2 mRNA and protein were detected by RT-qPCR and western blot, respectively. * P<0.05, ** P<0.01, versus control group; # P<0.05, ## P<0.01, versus Let-7g-5p mimics group; ^ P<0.05, ^^ P<0.01, versus IGF-1R group. RT-qPCR – real-time quantitative polymerase chain reaction.
Supplier Page from Abcam for Human IGF1 Receptor ELISA Kit