Fig 1: MiR-224-5p reduces airway inflammation in asthmatic mice through FHL1 suppression. Asthmatic mice were treated with lentivirus harboring miR-224-5p-agomir/NC-agomir alone or in combination with lentivirus harboring oe-FHL1/oe-NC. A Expression of miR-224-5p and FHL1 in lung tissues of asthmatic mice determined by RT-qPCR. B The airway resistance in asthmatic mice on the 1st day. C The airway resistance in asthmatic mice at various time points when the dosage of Mch is 200 mg/mL. D, The airway responsiveness in asthmatic mice. E The number of total cells (the left) and percentage of eosinophils in BALF of asthmatic mice. F Levels of IL-4, IL-5, IL-13 and IFN-? in BALF supernatant of asthmatic mice measured by ELISA. G HE staining images of lung tissues from asthmatic mice. H Masson’s trichrome staining images of lung tissues and collagen deposition area from asthmatic mice. * p < 0.05 compared with asthmatic mice treated with lentivirus harboring NC-agomir. # p < 0.05 compared with asthmatic mice treated with lentiviruses expressing miR-224-5p-agomir and oe-NC. n = 10 mice for each treatment
Fig 2: FHL1 expresses highly in mice with asthma-like airway inflammation. A The Venn map showing intersection of the top 200 DEGs obtained from asthma-related gene expression datasets GSE27066 and GSE6858. B The heatmap of the top 30 DEGs obtained from the GSE6858 dataset. The X axis represents the sample number, and the Y axis represents the DEGs; the upper right histogram is the color grade, where each rectangle corresponds to a sample expression value; the red indicates the high expression, and the green indicates the low expression. C The expression of FHL1 in asthma in the GSE27066 dataset. D Determination of airway responsiveness in mice with asthma-like airway inflammation. E HE staining images of bronchial cavities of similar diameter in lung tissues from normal and asthmatic mice. F The number of total cells and percentage of eosinophils in BALF of normal and asthmatic mouse lung tissues. G Levels of IL-4, IL-5, IL-13, and IFN-? in BALF supernatant of normal and asthmatic mouse lung tissues measured by ELISA. H Immunohistochemical images and quantitative analysis of FHL1 and VEGF positive expression in normal and asthmatic mouse lung tissues. * p < 0.05 compared with normal mice. n = 10 mice for each treatment
Fig 3: FHL1 down-regulation alleviates airway inflammation in mice with asthma-like airway inflammation. Normal mice served as the control and asthmatic mice were treated with lentivirus harboring oe-FHL1/oe-NC or sh-FHL1/sh-NC. A Expression of FHL1 in lung tissues of asthmatic mice determined by RT-qPCR. B, The airway resistance in asthmatic mice on the 1st day. C The airway resistance in asthmatic mice at various time points in response to Mch at a dosage of 200 mg/mL. D The airway responsiveness in asthmatic mice. E The number of total cells and percentage of eosinophils in BALF of asthmatic mice. F Levels of IL-4, IL-5, IL-13 and IFN-? in BALF supernatant of asthmatic mice measured by ELISA. G HE staining images of lung tissues of asthmatic mice. H Masson’s trichrome staining images of lung tissues and collagen deposition area of asthmatic mice. * p < 0.05 compared with normal mice. # p < 0.05 compared with asthmatic mice treated with lentivirus harboring sh-NC. $ p < 0.05 compared with asthmatic mice treated with lentivirus harboring oe-NC. n = 10 mice for each treatment
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