Fig 1: NACA improves cell survival in NPCs and ameliorates pathophysiological changes related to RILD. Radiation induced cell death and functional markers were measured at 3 days or 7 days post radiation injury. (A) Phase contrast image with DAPI staining to determine cell death at 3 days post 8 Gy radiation, hepatocytes (upper) and NPCs (lower). Briefly, nuclei were counted 3 days after radiation in 4 areas across 4 chips, one-way ANOVA with Tukey’s test, significance at p < 0.05, F(HEP) = 1.647, F(NPCs) = 102.1, DF = 2. (B) LDH effluent measured by ELISA for hepatocytes (left) and NPCs (right). One-way ANOVA with Tukey’s test was performed, significance at p < 0.05, F(HEP) = 2.58, F(NPCs) = 35.52, DF = 2. (C) CD31 is decreased in irradiated samples compared to 8 Gy + NACA, 3 days after radiation. CD31 fluorescence was measured across 3 areas in 3 chips, one-way ANOVA with Tukey’s test, significance at p < 0.05, F = 15.56, DF = 2. (D) α-SMA is increased in irradiated samples compared to 8 Gy + NACA + radiation. 3 days after radiation α-SMA was measured in 5 areas across 3 chips and normalized to cell number (DAPI), one-way ANOVA with Tukey’s test, significance at p < 0.05, F = 8.867, DF = 2. (E) depicts ELISA assays that were performed using NPCs lysates 3 days post radiation injury. These included VCAM-1, ICAM-1, SAA and CRP which were measured in 3 chips, as well as IL-6, Thrombomodulin, PDGFB, level were measured in 4 chips. One-way ANOVA with Tukey’s test was performed for all ELISAs, significance at p < 0.05, F(VCAM-1) = 6.128, F(ICAM-1) = 5.132, F(SAA) = 9.053, F(CRP) = 10.22, F(IL-6) = 8.9, F(Thrombomodulin) = 9.339, F(PDGFB) = 10.84, DF = 2. (F) show RT-PCR validation of TGFB1, TIMP1, TLR4 and CCN2 mRNA levels in NPCs 3 days after radiation, n = 3, one-way ANOVA with Tukey’s test, significance at p < 0.05, F(TGFB1) = 10.61, F(TIMP1) = 24.16, F(TLR4) = 19.19, F(CCN2) = 15.33, DF = 2. (G) depicts ELISA results from hepatocytes. Briefly TG, Urea and albumin were measured 3 days after radiation injury. TG utilized cell lysate while the Urea and Albumin assays relied on effluent. Results are from 4 chips, one-way ANOVA with Tukey’s test, significance at p < 0.05, F (TG) = 8.625, F(Urea) = 6.692, F(Alb) = 0.45, DF = 2. (G) miRNA RT-PCR for hepatocytes relevant to radiation damage. RT-PCR was performed 7 days post radiation injury, n = 3, ANOVA with Tukey’s test, F(miR-34a-5p) = 19.83, F(miR-122-5p) = 6.859, F(miR-21-5p) = 4.634, DF = 2.