Fig 1: Concentration of pro-inflammatory cytokines by ELISA in the serum samples of patients with MDD (n = 10) and healthy controls (n = 10). Concentrations of (A) IL-1ß; (B) IL-2; (C) IL-6; (D) TNF-a; (E) IFN-?; and (F) G-CSF. The concentration of pro-inflammatory cytokines in the serum of MDD patients was significantly increased. The experiments were repeated three times. The results are expressed as mean ± SD. *p < 0.05 vs. control group. MDD: Major depressive disorder; IL: Interleukin; TNF-a: Tumor necrosis factor alpha; IFN-?: Interferon gamma; G-CSF: Granulocyte colony-stimulating factor; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase; NF-?B: Nuclear factor kappa-light-chain-enhancer of activated B cells; ELISA: Enzyme-linked immunosorbent assay.
Fig 2: Concentration of pro-inflammatory cytokines by ELISA in the cultured medium of PC12 cells treated with corticosterone. Concentrations of (A) IL-1ß; (B) IL-2; (C) IL-6; (D) TNF-a; (E) IFN-?; and (F) G-CSF. The concentration of pro-inflammatory cytokines was decreased in KI vs. KT group. The experiments were repeated three times. The results are expressed as mean ± SD. *p < 0.05 vs. NC group. #p < 0.05 vs. KT group. NC: No treatment control group; KT: Ketamine treatment; KI: Ketamine treatment with the inhibition of NF-?B; KO: Ketamine treatment with the overexpression of NF-?B. IL: Interleukin; TNF-a: Tumor necrosis factor alpha; IFN-?: Interferon gamma; G-CSF: Granulocyte colony-stimulating factor; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase; NF-?B: Nuclear factor kappa-light-chain-enhancer of activated B cells; ELISA: Enzyme-linked immunosorbent assay.
Fig 3: Expression of pro-inflammatory cytokines by RT-qPCR in PC12 cells treated with corticosterone. Expression levels of (A) IL-1ß; (B) IL-2; (C) IL-6; (D) TNF-a; (E) IFN-?; and (F) G-CSF. The levels of pro-inflammatory cytokines were significantly decreased in KI vs. KT group. The experiments were repeated three times. The results are expressed as mean ± SD. *p < 0.05 vs. NC group. #p < 0.05 vs. KT group. GAPDH was used as the internal control. NC: No treatment control group; KT: Ketamine treatment; KI: Ketamine treatment with the inhibition of NF-?B; KO: Ketamine treatment with the overexpression of NF-?B. IL: Interleukin; TNF-a: Tumor necrosis factor alpha; IFN-?: Interferon gamma; G-CSF: Granulocyte colony-stimulating factor; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase; NF-?B: Nuclear factor kappa-light-chain-enhancer of activated B cells; RT-qPCR: Reverse transcription quantitative PCR.
Supplier Page from Abcam for Human G-CSF ELISA Kit