Fig 1: ELVN-34 reduce the expression of pro-inflammatory cytokines, chemokines and cell adhesion molecule in HNEpC challenged with LPS or poly(I:C). HNEpC challenged with LPS or poly(I:C) (30 µg/mL) display enhanced production of pro-inflammatory cytokines and chemokines IL-6, IL-1ß, IL-8/CXCL8, CCL2/MCP-1, CXCL1/KC/GRO, VEGF, and cell adhesion molecule ICAM1(CD54) compared to non-treated cells. This increase in the production of pro-inflammatory molecules is abrogated by the addition of ELVN-34 (500 nM) 30 min post-challenge. LPS and poly(I:C) induce a reduction of anti-inflammatory IL-10 expression in HNEpC that is restored to normal levels after treatment with ELVN-34 (500 nM). The results showed the averages of three independent experiments. (**** p < 0.0001, NS—not significant).
Fig 2: ELVN-34 reduce the expression of pro-inflammatory cytokines, chemokines, and cell adhesion molecule in HNEpC challenged with D. farinae, D. pteronyssinus, and HDM (D. fari + D. ptero) (30 µg/mL). ELVN-34 reduce the expression of pro-inflammatory cytokines, chemokines, and cell adhesion molecule ICAM1 in HNEpC exposed to HDM inducers. A remarkable decrease was observed in the release of anti-inflammatory cytokine IL-10 in HNEpC challenged with the HDM stressors (D. farinae, D. pteronyssinus, and HDM) (30 µg/mL). This decreased production of anti-inflammatory cytokine is reversed by the addition of ELVN-34 (500 nM), 30 min post-challenge with the stressor. The results showed the averages of three independent experiments. (**** p < 0.0001).
Supplier Page from Abcam for Human ICAM1 ELISA Kit (CD54)