Human IgG ELISA Kit ab100547 from Abcam

Product Specs
ItemHuman IgG ELISA Kit
CompanyAbcam
Price $529.00Supplier Page View Company Product Page
Catalog Numberab100547
MethodELISA
Quantity1 x 96 Tests
ApplicationssELISA, ELISA
Gene NameIghg1
Detection Range0.02 - 15 ng/ml
SensitivityLess than 20 pg/mL

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) ACE-2-like Enzymatic Activity in Anti-SARS-CoV-2 Spike Protein Monoclonal AntibodiesbioRxivFebruary 3, 2026Yufeng Song, Frances Mehl, Tom No, Lauren Livingston, Juan Sebastian Quintero Quintero Barbosa, Jun Hayashi, Ginette Serrero, Pamela Schoppee Bortz, Jeffrey M Wilson, James E Crowe, et al.quantitative human IgG ELISA following the kit manufacturer’s instructions (Abcam, Waltham, MA. Cat. #ab100547) as described26, 27. Data Analysis ACE2 substrate cleavage was monitored by measuring RFU for 16 hours

(2) ACE-2-like enzymatic activity in COVID-19 convalescents with persistent pulmonary symptoms associated with immunoglobulinmBioAugust 13, 2025Yufeng Song, Frances Mehl, Lyndsey M. Muehling, Glenda Canderan, Kyle Enfield, Jie Sun, Michael T. Yin, Sarah J. Ratcliffe, Jeffrey M. Wilson, Alexandra Kadl, et al.to further deplete the antibodies. Human IgG was measured using a Human IgG ELISA Kit (Abcam, Cat. #ab100547) according to the manufacturer’s protocol as described (43). Briefly, 100 μL samples, as well as standards,

(3) ACE-2-like enzymatic activity is associated with immunoglobulin in COVID-19 patientsmBioApril 10, 2024Song Y, Myers R, Mehl F, Murphy L, Brooks B, Wilson JM, Kadl A, Woodfolk J, Zeichner SL.Human IgG was measured using a human IgG enzyme-linked immunosorbent assay (ELISA) kit (Abcam, Cat. #ab100547) according to the manufacturer’s protocol. We added 50 µL processed samples, as well as standards to

(4) Production of recombinant human IgG1 Fc with beneficial N-glycosylation pattern for anti-inflammatory activity using genome-edited chickensCommunications biologyJune 1, 2023Park JS, Choi HJ, Jung KM, Lee KY, Shim JH, Park KJ, Kim YM, Han JY.collected serum and yolk samples were diluted in DW and quantified by ELISA using a human IgG ELISA kit (ab100547, Abcam, Cambridge, UK), according to manufacturer’s instruction. The measured concentrations were divided

(5) The characters of antibodies against PLA2R in healthy individuals and in the patient with PLA2R associated membranous nephropathyEuropean Journal of Medical ResearchMarch 20, 2023Yan-jiao Cheng, Miao Wang, Jia Wang, Zhao Cui, Ming-hui Zhaoeach subclass/the total OD value × 100%. The IgG concentration was measured by a commercial ELISA kit (ab100547, Abcam, Cambridge, UK) for human IgG detection. The procedure was performed according to the instructions.

(6) Sialylation of IgG inhibits the formation of galactose-deficient IgA1-containing immune complexes and protects mesangial cells from injury in IgA nephropathyBMC NephrologyDecember 1, 2022Youxia Liu, Hongfen Li, Huyan Yu, Fanghao Wang, Junya Jia, Tiekun Yanplasma The level of SA-IgG in plasma was determined using ELISA according the slightly modified procedure (ab100547; Abcam, UK). Briefly, 100 μL of diluted plasma was added into each well and incubated for 2.5-hs at

(7) Immunology, Treatment and Public Health Aspects of Subarachnoid HemorrhageIran. J. Public HealthJune 18, 2020Junjing Zhao, Jianping Zhang, Yongxia Bu, Wei Lu, Gejin ZhaoInstruments and Reagents Ig G protein, Ig M protein, Ig A protein ELISA kits (Shanghai Abcam Company, China, ab100547, ab137982, ab196263). Methods of Treatment Interventional embolization of aneurysms was performed

(8) Downregulated miR‑29a promotes B cell overactivation by upregulating Crk‑like protein in systemic lupus erythematosusMolecular Medicine ReportsMay 21, 2020Xinyu Shi et al.concentration in the B cell culture supernatant was determined using a human IgG ELISA kit (cat. no. ab100547; Abcam), according to the manufacturer's protocol. All experiments were performed in triplicate. The

(9) Bevacizumab suppresses the growth of established non-small-cell lung cancer brain metastases in a hematogenous brain metastasis modelClinical & Experimental MetastasisFebruary 1, 2020Chinami Masuda et al.according to the manufacturer’s instruction using a Human VEGF ELISA Kit (ab100663) and a HuIgG ELISA Kit (ab100547) (Abcam plc, Cambridge, UK), respectively. Benchmark Plus Microplate Reader (Bio-Rad Laboratories, Inc.,

(10) Human monoclonal antibodies against chikungunya virus target multiple distinct epitopes in the E1 and E2 glycoproteinsPLOS PathogensNovember 7, 2019Jose A. Quiroz et al.ELISA to detect human IgG. Mouse serum samples were tested using a commercial ELISA (Abcam cat No. ab100547) for quantifying human IgG. Data was analyzed and graphed using GraphPad Prism v6.0. Negative stain

(11) Tumor-derived Autoantibodies Identify Malignant Pulmonary NodulesAmerican Journal of Respiratory and Critical Care MedicineMay 15, 2019Kristin J. Lastwika et al.harvested and stored at 2808 C. ELISA IgG and IgM levels were determined using ELISA kits (Abcam: IgG, ab100547; IgM, ab137982) on matched plasma and BCE following manufacturer’s instructions. Free Autoantibody Detection Free

(12) Isolation, Detection, and Quantification of Cancer Biomarkers in HPV-Associated MalignanciesScientific reportsJune 12, 2017Inan H, Wang S, Inci F, Baday M, Zangar R, Kesiraju S, Anderson KS, Cunningham BT, Demirci U.was obtained from Life Technologies (Grand Island, NY). Human IgG ELISA Kit was purchased from Abcam (ab100547) (Cambridge, MA). Whole Blood (EDTA) was obtained from Stanford Blood Center (Palo Alto, CA). 3,3′,5,5′-Tetramethylbenzidine

(13) Differential Immune Reactivity Pattern of SW48 and SW1116 Colorectal Cancer Cell Lines with Colorectal Cancer Patients SeraAdvanced Biomedical ResearchJanuary 31, 2017Ghasem Ghalamfarsa, Seyyed Vahid Hosseini, Maryam Hamidinia, Abbas Ghaderi, Mahmoud Mahmoudi, Zahra Mojtahedi

(14) Measuring the Change in Zinc Ion Concentration in Eye Tear Fluid between Healthy and Parasite Infected Individuals: Relationship between Zinc Ions in Tear Fluid and Parasitic Infection by Soil-Transmitted HelminthsJournal of Medical Diagnostic MethodsJanuary 1, 2016Framroze Z, Conroy Cimmediately. For information on reagent preparation, see Abnova KA0216 Human (IgE) ELISA Assay kit and Abcam: ab100547 – IgG Human ELISA Kit. Results The data from this study was analysed in two stages. First, we performed

(15) Quantitative evaluation of the immunodeficiency of a mouse strain by tumor engraftmentsJournal of Hematology & OncologyDecember 1, 2015Wei Ye et al.Enzyme-linked immunosorbent assay The human Ig concentration in recipient serum was measured by using a human Ig assay kit (ab100547, Abcam, Cambridge, UK). To detect OVA-specific human IgG antibodies, five recipient BLT mice were immunized

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Human IgG ELISA Kit from Abcam

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Human IgG ELISA Kit from Abcam

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