Description
This is a colorimetric competitive immunoassay kit for the quantitative determination of extracellular cGMP diluted in buffer or intracellular cGMP in cells or tissues lysed in 0.1 M HCl. 0.1M HCl is used to stop endogenous phosphodiesterase activity and stabilize the released cyclic GMP. The 0.1M HCl treated samples are then analyzed directly in a microtiter plate without extraction, drying and reconstitution. Sensitivity is increased by acetylation (reagents included). Absorbance is read at 405 nm. The ready-to-use liquid color-coded reagents provided reduce error